LAMTOR1 蛋白, Human (His)

The LAMTOR1 protein is an important component of the Ragulator complex and plays dual roles in amino acid sensing and mTORC1 activation. It acts as a guanine nucleotide exchange factor (GEF) for Rag GTPases and mediates their recruitment to the lysosomal membrane. LAMTOR1 Protein, Human (His) is the recombinant human-derived LAMTOR1 protein, expressed by E. coli , with N-His labeled tag. The total length of LAMTOR1 Protein, Human (His) is 160 a.a., with molecular weight of ~24 kDa.

LAMTOR1 是 Ragulator 复合体中的重要组成部分，Ragulator 复合体是一种涉及氨基酸传感和 mTORC1 激活的多方面组件，mTORC1 是一种信号连接，协调细胞生长以响应不同的刺激，包括生长因子、能量水平和氨基酸。Ragulator 对小 GTPases Rag 具有双重作用，既充当激活 Rag GTPases 的鸟嘌呤核苷酸交换因子 (GEF)，又充当将其募集至溶酶体膜的介质。在这种复杂的相互作用中，激活的 Ragulator 和 Rag GTPases 协同充当支架，协调 mTORC1 在溶酶体的招募和激活。LAMTOR1 是这一机制的关键，它直接将 Ragulator 复合物锚定在溶酶体膜上，在将 Ragulator 复合物的其他亚基结合在一起方面发挥着关键作用。此外，LAMTOR1 与 Rag GTPases 的相互作用在 mTORC1 复合物向溶酶体的募集中发挥着核心作用。除了在 mTOR 信号传导中的作用之外，LAMTOR1 还通过参与控制胚胎干细胞分化、调节晚期内体/溶酶体生物合成以及潜在参与胆固醇稳态和 RHOA 激活等过程来展示其多功能性。作为 Ragulator 复合体和各种相互作用网络的一部分，LAMTOR1 作为细胞对不同刺激做出反应的关键协调器而出现，有助于调节基本的细胞过程。

Human

E. coli

N-His

Q6IAA8 (2G-161P)

55004  [NCBI]

GCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLASEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.