1. 重组蛋白
  2. Receptor Proteins
  3. LDLR 蛋白, Mouse (Biotinylated, A23V, C27G, HEK293, His)

LDLR 蛋白, Mouse (Biotinylated, A23V, C27G, HEK293, His)

目录号: HY-P73838
产品使用指南

LDLR 蛋白与 LDL 关键结合,通过内吞作用转运胆固醇。聚集成网格蛋白包被的小凹坑启动内化。与 DAB2、LDLRAP1、ARRB1、SNX17 和不成熟的 PCSK9 的相互作用有助于其功能的多功能性。酪氨酸磷酸化会损害 NPXY 基序相互作用。LDLR 蛋白, Mouse (Biotinylated, A23V, C27G, HEK293, His) 是重组的 LDLR 蛋白,由 HEK293 表达,带有 C-His 标签。 和 A23V,C27G 突变。

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描述

LDLR Protein crucially binds to LDL, transporting cholesterol through endocytosis. Clustering into clathrin-coated pits initiates internalization. Interactions with DAB2, LDLRAP1, ARRB1, SNX17, and immature PCSK9 contribute to its functional versatility. The NPXY motif interaction is impaired by tyrosine phosphorylation. LDLR Protein, Mouse (Biotinylated, A23V, C27G, HEK293, His) is the recombinant mouse-derived LDLR protein, expressed by HEK293 , with C-His labeled tag and A23V, C27G mutation.

研究背景

LDLR 蛋白在与 LDL 结合方面发挥着至关重要的作用,LDL 是负责在血液中运输胆固醇的主要脂蛋白,并通过内吞作用促进其内化到细胞中。为了进行内化,受体-配体复合物必须首先聚集成网格蛋白包被的凹坑。此外,LDLR 通过其 NPXY 基序与多种蛋白质(包括 DAB2 和 LDLRAP1)相互作用,且这种相互作用会因 NPXY 基序的酪氨酸磷酸化而受损。它还与 ARRB1 和 SNX17 相互作用,进一步增强了其功能的多功能性。此外,LDLR 与未成熟形式的 PCSK9 相互作用,有助于调节 LDL 胆固醇水平。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P35951/NP_034830.2 (A22-R790, A23V, C27G)

基因 ID
蛋白结构
N-term
LDLR (A22-R790, A23V, C27G)
Accession # P35951/NP_034830.2
His
C-term
同用名
Low-density lipoprotein receptor; LDLR; LDL Receptor
分子量

Approximately 85.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LDLR Protein, Mouse (Biotinylated, A23V, C27G, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LDLR Protein, Mouse (Biotinylated, A23V, C27G, HEK293, His)
目录号:
HY-P73838
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