LEDGF p52 蛋白, Human

LEDGF p52 protein is involved in essential functions, such as DNA-binding and chromatin binding activity. It positively regulates transcription and plays a role in mRNA 5'-splice site recognition. The protein is located in heterochromatin, the nuclear periphery, and the nucleoplasm. It is ubiquitously expressed in various tissues, including the brain (RPKM 41.2), ovary (RPKM 33.4), and 24 other tissues. LEDGF p52 Protein, Human is the recombinant human-derived LEDGF p52 protein, expressed by E. coli , with tag free.

LEDGF p52 蛋白参与多种基本功能，包括 DNA 结合转录因子结合活性、染色质结合活性和转录共激活子活性。它在 mRNA 5'-剪接位点识别中发挥作用，并通过 RNA 聚合酶 II 正向调节转录。该蛋白位于异染色质、核周边和核质中。它在多种组织中普遍表达，包括大脑 (RPKM 41.2)、卵巢 (RPKM 33.4) 和 24 种其他组织。（参考：基因组资源联盟提供，2022年4月）

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 5.323 μg/mL.

Human

E. coli

Tag Free

O75475-2/NP_066967 (M1-Q333)

11168  [NCBI]

MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEHQTTCNLQ

Approximately 51 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.