Leptin 蛋白, Rat (His)

Leptin Protein, Rat is a hormone secreted by fat cells that helps to regulate energy balance by inhibiting feeding and increase thermogenesis.

传感器（脂肪细胞产生的瘦素）监测脂肪组织块的大小。下丘脑中心通过瘦素受体（LRb）接收并整合瘦素信号的强度。效应系统（包括交感神经系统）控制着能量平衡的两个主要决定因素——能量摄入和能量消耗^[1]。瘦素基因的隐性突变与小鼠和人类的严重肥胖有关，为肥胖奠定了遗传基础。瘦素在血液中循环并作用于大脑以调节食物摄入和能量消耗。当脂肪量下降时，血浆瘦素水平下降，刺激食欲并抑制能量消耗，直到脂肪量恢复。当脂肪量增加时，瘦素水平就会增加，抑制食欲直到体重减轻。该系统维持脂肪组织质量的稳态控制^[2]。

Fully biologically active determined by the dose dependent proliferation of MCF7 cells. The ED₅₀ for this effect is ≤14 ng/mL, corresponding to a specific activity is ≥7.14×10⁴ units/mg.

• 
  Fully biologically active determined by the dose dependent proliferation of MCF7 cells. The ED₅₀ for this effect is 13.06 ng/mL, corresponding to a specific activity is 7.66×10⁴ units/mg.

Rat

E. coli

N-6*His

P50596 (V22-C167)

25608  [NCBI]

VPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC

Approximately 17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.