Leptin 蛋白, Rat

Leptin Protein, Rat is a hormone secreted by fat cells that helps to regulate energy balance by inhibiting feeding and increase thermogenesis.

传感器（脂肪细胞产生的瘦素）监测脂肪组织块的大小。下丘脑中心通过瘦素受体（LRb）接收并整合瘦素信号的强度。效应系统（包括交感神经系统）控制着能量平衡的两个主要决定因素——能量摄入和能量消耗^[1]。瘦素基因的隐性突变与小鼠和人类的严重肥胖有关，为肥胖奠定了遗传基础。瘦素在血液中循环并作用于大脑以调节食物摄入和能量消耗。当脂肪量下降时，血浆瘦素水平下降，刺激食欲并抑制能量消耗，直到脂肪量恢复。当脂肪量增加时，瘦素水平就会增加，抑制食欲直到体重减轻。该系统维持脂肪组织质量的稳态控制^[2]。

1.The ED₅₀ is <10 μg/mL as measured by LoVo cells, corresponding to a specific activity of >100 units/mg.
2.Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human Leptin R. The ED₅₀ for this effect is 0.2792 ng/mL, corresponding to a specific activity is 3.582×10⁶ units/mg.

Rat

E. coli

Tag Free

P50596 (V22-C167)

25608  [NCBI]

VPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGLDFIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC

Approximately 16.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jéquier E, et al. Leptin signaling, adiposity, and energy balance. Ann N Y Acad Sci. 2002 Jun;967:379-88.  [Content Brief]

  [2]. Friedman JM, et al. Leptin and the regulation of body weigh. Keio J Med. 2011;60(1):1-9.  [Content Brief]