Leukocyte elastase inhibitor/SERPINB1 蛋白, Pig (His, myc)

The leukocyte elastase inhibitor/SERPINB1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) is the recombinant pig-derived Leukocyte elastase inhibitor/SERPINB1 protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) is 378 a.a., with molecular weight of ~50.0 kDa.

白细胞弹性蛋白酶抑制剂/SERPINB1 蛋白是一种中性粒细胞丝氨酸蛋白酶抑制剂，在调节先天免疫反应、炎症和细胞稳态中发挥着关键作用。其主要功能是保护细胞免受应激或感染期间细胞质中释放的蛋白酶的影响，防止颗粒释放的强效酶造成的潜在伤害。SERPINB1 有效调节中性粒细胞蛋白酶，包括弹性蛋白酶、组织蛋白酶 G、蛋白酶-3、糜酶、胰凝乳蛋白酶和激肽释放酶-3。此外，它是 GZMH 的有效细胞内抑制剂，直接阻碍其蛋白水解活性。在炎症过程中，SERPINB1 通过抑制炎症性半胱天冬酶（CASP1、CASP4 和 CASP5）的半胱天冬酶募集结构域 (CARD) 寡聚化和酶激活来降低其活性。当分泌时，SERPINB1 通过其蛋白酶抑制功能促进 β 细胞增殖。它可能会发生裂解，导致抗蛋白酶活性丧失并出现核酸内切酶活性，但尚未确定具体的催化位点。

Pig

E. coli

N-His;C-Myc

P80229 (M1-P378)

100155145  [NCBI]

MEQLSAANTRFALDLFRALNESNPAGNIFISPFSISSALAMILLGTRGNTEAQMSKALHFDTVKDIHSRFQSLNADINKCGASYILKLANRLFGEKTYHFLPEFLASTQKTYGAELASVDFLRASEEARKAINEWVKEQTEGKIPELLASGVVDSATKLVLVNAIYFKGSWQEKFMTEATKDAPFRLNKKDSKTVKMMYQKKKFPFGYIKELKCRVLELPYQGKDLSMVILLPDSIEDESTGLRKIEQHLTLEKLNEWTKPDNLELLEVNVHLPRFRLEESYDLNAPLARLGVQDLFGSRADLTGMSEARDLFISKVVHKAFVEVNEEGTEAAAATAGIAVFAMLMPEEDFIADHPFIFFIRHNPSSNILFLGRLSSP

Approximately 50.0 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.