LIF 蛋白, Mouse

LIF Protein, Mouse is a lymphoid factor involved in various neural and inflammatory processes such as the acute-phase reaction, tissue damage, and infection.

白血病抑制因子 (LIF) 已被证明具有多种显着的作用。它从骨组织中释放钙，是阻止正常胚胎干细胞自发分化的分化抑制因子^[1]。白血病抑制因子（LIF）参与对损伤的神经和免疫反应。在多种动物和人类炎症条件下，其水平会升高。在某些情况下，例如气管内脂多糖诱导的炎症后，服用白血病抑制因子可以抑制炎症症状。白血病抑制因子还通过下丘脑-垂体-肾上腺轴增加皮质酮水平。外源添加的白血病抑制因子可诱导急性期蛋白表达并刺激促炎细胞因子和单核细胞趋化剂的产生^[2]。在造血系统中，LIF诱导某些白血病细胞的分化以及造血干细胞、巨核祖细胞和DA1细胞的增殖。LIF 还具有骨重塑、诱导肝细胞急性期反应、抑制脂肪生成、调节神经分化和抑制肾上皮细胞分化的活性^[3]。

1.The ED₅₀ is <0.01 ng/mL as measured by M1 cells, corresponding to a specific activity of >1.0 × 10⁸ units/mg.
2.Measured by its ability to induce IL-6 secretion by M-NFS-60 mouse cells. The ED₅₀ for this effect is 2.407 ng/mL, corresponding to a specific activity is 4.15×10⁵ U/mg.
3.Measured in a cell proliferation assay using C2C12 cell. The ED₅₀ for this effect is 0.3801-0.412 ng/mL.

• 
  Measured by its ability to induce IL-6 secretion by M-NFS-60 mouse cells.The ED₅₀ for this effect is 2.407 ng/mL, corresponding to a specific activity is 4.15×10⁵ U/mg.

Mouse

E. coli

Tag Free

P09056 (S24-F203)

16878  [NCBI]

SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAFSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPF

Approximately 20 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0 or 20mM PB, 150mM NaCl, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCL, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Metcalf D, et al. Effects of injected leukemia inhibitory factor on hematopoietic and other tissues in mice. Blood. 1990 Jul 1;76(1):50-6.  [Content Brief]

  [2]. Banner LR, et al. Leukemia inhibitory factor is an anti-inflammatory and analgesic cytokine. J Neurosci. 1998 Jul 15;18(14):5456-62.  [Content Brief]

  [3]. Gearing DP, et al. Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130. EMBO J. 1991 Oct;10(10):2839-48.  [Content Brief]