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  3. Lipocalin-2/NGAL 蛋白, Rat (sf9, His)

Lipocalin-2/NGAL 蛋白, Rat (sf9, His)

目录号: HY-P74774
COA 产品使用指南

Lipocalin-2/NGAL 是一种多方面的铁转运蛋白,参与多种生物过程,包括细胞凋亡、先天免疫和肾脏发育。Lipocalin-2 通过与铁载体 2,3-二羟基苯甲酸酯结合动态影响细胞铁浓度。Lipocalin-2/NGAL 蛋白, Rat (sf9, His) 是重组的 Lipocalin-2/NGAL 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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规格 价格 是否有货 数量
5 μg ¥650 In-stock
10 μg ¥1050 In-stock
20 μg ¥1700 In-stock
50 μg ¥3200 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Lipocalin-2/NGAL is a multifaceted iron transporter involved in multiple biological processes, including apoptosis, innate immunity, and kidney development. Lipocalin-2 dynamically affects cellular iron concentration through binding to the siderophore 2,3-dihydroxybenzoate. Lipocalin-2/NGAL Protein, Rat (sf9, His) is the recombinant rat-derived Lipocalin-2/NGAL protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

Lipocalin-2/NGAL 是一种多方面的铁转运蛋白,参与多种生物过程,包括细胞凋亡、先天免疫和肾脏发育。通过与铁载体 2,3-二羟基苯甲酸 (2,3-DHBA) 的结合,Lipocalin-2 结合并穿梭铁,动态影响基于 Holo-24p3(铁结合)或 apo-24p3(铁)的细胞铁浓度。-免费)表格。与 SLC22A17 受体的相互作用介导铁释放或螯合,精细调节细胞内铁水平。在白细胞介素 3 (IL3) 剥夺引发的细胞凋亡中,含铁形式可防止细胞凋亡,而无铁形式则诱导 BCL2L11/BIM 表达,导致程序性细胞死亡。Lipocalin-2 还通过隔离细菌铁载体(例如肠杆菌素)来促进先天免疫,并表现出结合结核分枝杆菌铁载体的能力。在结构上,Lipocalin-2 以单体、同二聚体(二硫键连接)形式存在,并与 MMP9 形成异二聚体(二硫键连接)。这些不同的相互作用强调了 Lipocalin-2 在协调关键细胞和免疫反应方面的多功能性。

生物活性

Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin2 . It binds >1.0 μM of Fe(DHBA)3.

种属

Rat

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P30152 (Q21-N198)

基因 ID
蛋白结构
N-term
NGAL (Q21-N198)
Accession # P30152
His
C-term
同用名
Neutrophil gelatinase-associated lipocalin; NGAL; Lipocalin 24p3; p25; LCN2
分子量

Approximately 25 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Lipocalin-2/NGAL Protein, Rat (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Lipocalin-2/NGAL Protein, Rat (sf9, His)
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HY-P74774
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