LOK 蛋白, Human (His)

LOK is an important serine/threonine protein kinase that significantly regulates lymphocyte migration by phosphorylating MSN and potentially PLK1. It acts as a negative regulator of MAP3K1/MEKK1. LOK Protein, Human (His) is the recombinant human-derived LOK protein, expressed by E. coli , with N-His labeled tag. The total length of LOK Protein, Human (His) is 300 a.a., with molecular weight of ~40 kDa.

LOK 是一种丝氨酸/苏氨酸蛋白激酶，在淋巴细胞迁移的调节中发挥着重要作用。它通过磷酸化 MSN 和潜在的 PLK1 等关键靶点来实现这一目标，同时还充当 MAP3K1/MEKK1 的负调节因子。LOK 在淋巴细胞迁移的协调中的参与尤其值得注意，因为它介导 ERM 蛋白的磷酸化，以 MSN 为例。此外，LOK 可能会扩大其作为细胞周期调节剂的影响，可能发挥 polo 激酶激酶的作用，正如其在体外磷酸化 PLK1 的能力所表明的那样；然而，这种体内调节作用的确认还有待更多的经验证据。

The specific activity was determined to be >200 nmol/min/mg using synthetic AXLtide peptide (KKSRGDYMTMQIG) as substrate.

Human

E. coli

N-His

O94804 (R18-E317)

6793  [NCBI]

Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.