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  3. Lymphocyte antigen 6H/LY6H 蛋白, Human (HEK293, His)

Lymphocyte antigen 6H/LY6H 蛋白, Human (HEK293, His)

目录号: HY-P70217
产品使用指南

LY6H 通过 GPR37 和 GPR37L1 受体介导 PSAP/Prosaposin 蛋白的髓磷脂营养和神经营养作用。这些受体的配体介导的内化导致 ERK 磷酸化信号传导。Lymphocyte antigen 6H/LY6H 蛋白, Human (HEK293, His) 是重组的 淋巴细胞抗原 6H/LY6H 蛋白,由 HEK293 表达,带有 C-6*His 标签。Lymphocyte antigen 6H/LY6H 蛋白, Human (HEK293, His) 全长 90 个氨基酸,分子量约为 ~18.0 kDa。

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描述

LY6H mediates the myelin trophic and neurotrophic effects of PSAP/Prosaposin protein through GPR37 and GPR37L1 receptors. Ligand-mediated internalization of these receptors results in ERK phosphorylation signaling. Lymphocyte antigen 6H/LY6H Protein, Human (HEK293, His) is the recombinant human-derived Lymphocyte antigen 6H/LY6H protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Lymphocyte antigen 6H/LY6H Protein, Human (HEK293, His) is 90 a.a., with molecular weight of ~18.0 kDa.

研究背景

PSAP/Prosaposin 蛋白作为髓鞘营养因子和神经营养因子,通过 G 蛋白偶联受体 GPR37 和 GPR37L1 发挥作用,这些受体经历配体介导的内化,然后进行 ERK 磷酸化信号传导。此外,PSAP 的成分 saposin-A 和 saposin-C 通过各自的酶刺激葡萄糖神经酰胺和半乳糖神经酰胺的水解。值得注意的是,saposin-C 被认为与酶和酸性脂质结合形成活化复合物,这表明其作用机制涉及复合物形成而不是底物溶解。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

O94772 (L26-G115)

基因 ID
蛋白结构
N-term
LY6H (L26-G115)
Accession # O94772
6*His
C-term
同用名
rHuLymphocyte antigen 6H/LY6H, His; Lymphocyte Antigen 6H; Ly-6H; LY6H
氨基酸序列

LWCQDCTLTTNSSHCTPKQCQPSDTVCASVRITDPSSSRKDHSVNKMCASSCDFVKRHFFSDYLMGFINSGILKVDVDCCEKDLCNGAAG

分子量

Approximately 18.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Lymphocyte antigen 6H/LY6H Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Lymphocyte antigen 6H/LY6H Protein, Human (HEK293, His)
目录号:
HY-P70217
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