M-CSF 蛋白, Rat (HEK293)

The M-CSF protein regulates hematopoietic precursor cells, promoting their survival, proliferation, and differentiation. M-CSF Protein, Rat (HEK293) is the recombinant rat-derived M-CSF protein, expressed by HEK293 , with tag free.

M-CSF 蛋白在协调造血前体细胞（特别是单核吞噬细胞，包括巨噬细胞和单核细胞）的生存、增殖和分化的调节中发挥着至关重要的作用。其重要性延伸到促进促炎趋化因子的释放，从而在先天免疫和炎症过程中发挥关键作用。此外，M-CSF 在调节破骨细胞增殖和分化、影响骨吸收并有助于正常骨发育方面发挥着关键作用。除了对骨骼系统的影响之外，M-CSF 对于正常男性和女性的生育能力也是不可或缺的。细胞因子还在脂蛋白清除中发挥作用，并有助于细胞过程，例如重组肌动蛋白细胞骨架、调节膜皱褶的形成、细胞粘附和细胞迁移。M-CSF可以多种形式存在，包括具有两个相同的150-200kDa蛋白聚糖亚基的同二聚体、具有150-200kDa蛋白聚糖亚基和截短的43kDa亚基的异二聚体、以及具有两个相同的43kDa亚基的同二聚体。它与其受体 CSF1R 相互作用以发挥其多种功能。

Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ for this effect is 0.02302 ng/mL, corresponding to a specific activity is 4.34×10⁷ units/mg.

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  Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ for this effect is 0.02302 ng/mL, corresponding to a specific activity is 4.34×10⁷ units/mg.

Rat

HEK293

Tag Free

Q8JZQ0 (E33-R254)

78965  [NCBI]

EVSEHCSHMIGNGHLQILQQLIDSQMETACLIEYKFVDQEQLDDPVCYLKKAFVLVQVIIEETMRFKDNTPNANATERLQELSMKLNSCFIKDYKEQNEACVQTYKESPLRLLEKIKNFFNETKNFLEKDWNIFSKNCNDSFAKCSSRDVVTKPDCNCLYPKATPSSDLASASPHQPPAPSMAPLADLAWDDSQRTEGSSLLPSDLPLRIEDPGSAKQRPPR

Approximately 46.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.