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  3. MAP1LC3A 蛋白, Human (His)

MAP1LC3A 蛋白, Human (His)

目录号: HY-P70916
产品使用指南

MAP1LC3A 是自噬体液泡形成所必需的关键类泛素修饰剂,可确保细胞稳态。作为 GABARAP/GATE-16 亚家族的一部分,它在自噬体晚期成熟中发挥着至关重要的作用。MAP1LC3A 蛋白, Human (His) 是重组的 MAP1LC3A 蛋白,由 E. coli 表达,带有 C-6*His 标签。

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描述

MAP1LC3A is a key ubiquitin-like modifier essential for autophagosome vacuole formation, ensuring cellular homeostasis. As part of the GABARAP/GATE-16 subfamily, it plays a crucial role in late autophagosome maturation. MAP1LC3A Protein, Human (His) is the recombinant human-derived MAP1LC3A protein, expressed by E. coli , with C-6*His labeled tag.

研究背景

MAP1LC3A 是一种泛素样修饰剂,在自噬体液泡形成中发挥着至关重要的作用,有助于细胞稳态。属于 GABARAP/GATE-16 亚家族的 MAP1LC3A 在参与吞噬细胞膜的伸长过程中,在自噬体成熟的后期阶段发挥着至关重要的作用。它参与将内质网子域重塑为自噬体以响应营养应激,促进其随后与溶酶体融合以实现内质网周转。它具有三种不同的轻链(LC1、LC2 和 LC3),可以与 MAP1A 和 MAP1B 蛋白结合。MAP1LC3A 表现出多样化的相互作用组,与 TP53INP1、TP53INP2、SQSTM1 等蛋白质(用于包涵体降解)、ATG13、ULK1、TBC1D5、UBQLN1、UBQLN2、UBQLN4、TRIM5、MEFV 等相互作用。这些相互作用强调了 MAP1LC3A 在各种细胞过程中的多方面作用,包括自噬、蛋白质聚集体降解和网状自噬的调节。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

Q9H492-1 (M1-F121)

基因 ID
蛋白结构
N-term
MAP1LC3A (M1-F121)
Accession # Q9H492-1
6*His
C-term
同用名
Microtubule-Associated Proteins 1A/1B Light Chain 3A; Autophagy-Related Protein LC3 A; Autophagy-Related Ubiquitin-Like Modifier LC3 A; MAP1 Light Chain 3-Like Protein 1; MAP1A/MAP1B Light Chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-Associated Protein 1 Light Cha
氨基酸序列

MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF

分子量

Approximately 16.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MAP1LC3A Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MAP1LC3A Protein, Human (His)
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