1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2) Serine/Threonine Kinase Proteins Protein Tyrosine Kinases
  4. MAP3K1 蛋白, Mouse (His)

MAP3K1 蛋白, Mouse (His)

目录号: HY-P700593
产品使用指南

MAP3K1 是蛋白激酶信号转导级联的关键组成部分,在细胞信号传导通路中发挥着至关重要的作用。通过其激酶活性,MAP3K1 通过磷酸化 MAP2K1 和 MAP2K4 来协调 ERKJNK 激酶途径的激活。MAP3K1 蛋白, Mouse (His) 是重组的 MAP3K1 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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描述

MAP3K1 is a key component of the protein kinase signal transduction cascade and plays a crucial role in cell signaling pathways.Through its kinase activity, MAP3K1 coordinates the activation of the ERK and JNK kinase pathways by phosphorylating MAP2K1 and MAP2K4.MAP3K1 Protein, Mouse (His) is the recombinant mouse-derived MAP3K1 protein, expressed by E.coli , with N-6*His labeled tag.

研究背景

MAP3K1(丝裂原激活蛋白激酶激酶 1)是蛋白激酶信号转导级联的关键组成部分,在细胞信号转导中发挥着关键作用。它通过 MAP2K1 和 MAP2K4 磷酸化激活 ERKJNK 激酶途径发挥作用。此外,MAP3K1 可能磷酸化 MAPK8/JNK1 激酶,进一步促进细胞反应的调节。此外,MAP3K1 能够激活 NF-kappa-B 通路中的核心蛋白激酶 CHUK 和 IKBKB,表明其参与免疫和炎症反应的调节。MAP3K1 的多方面作用凸显了其在协调复杂信号网络中的重要性,使其成为各种细胞过程的重要调节者。(参考:提供的资料)

种属

Mouse

表达系统

E. coli

标签

N-6*His

蛋白编号

P53349 (Q1216-W1493)

基因 ID
蛋白结构
N-term
6*His
MAP3K1 (Q1216-W1493)
Accession # P53349
C-term
同用名
MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1; MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; mitogen-activated protein kinase kinase kinase 1
氨基酸序列

QPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQDRPPSRELLKHPVFRTTW

分子量

34.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MAP3K1 Protein, Mouse (His)
目录号:
HY-P700593
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