MASP1 蛋白, Human (HEK293, His)

MASP1 Protein, Human (HEK293, His) is a recombinant human Mannan-binding lectin serine protease 1 produced in HEK293 cells, with a His tag at the C-terminus. Mannan-binding lectin serine protease 1 is a serine protease associated with humoral pattern-recognition molecules^[1].

甘露聚糖结合凝集素 (MBL) 相关丝氨酸蛋白酶（MASP-1、-2 和 -3）是同源模块化蛋白酶，各自与 MBL 以及 L-和 H-ficolins 相关，后者是参与先天免疫的寡聚血清凝集素。MASP-1 是一种与体液模式识别分子相关的丝氨酸蛋白酶，是补体凝集素途径的丰富成分。MASP-1 可以自动激活^[1][2]。

Human

HEK293

C-6*His

P48740-2 (H20-R728)

5648  [NCBI]

HTVELNNMFGQIQSPGYPDSYPSDSEVTWNITVPDGFRIKLYFMHFNLESSYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGSFMSITFRSDFSNEERFTGFDAHYMAVDVDECKEREDEELSCDHYCHNYIGGYYCSCRFGYILHTDNRTCRVECSDNLFTQRTGVITSPDFPNPYPKSSECLYTIELEEGFMVNLQFEDIFDIEDHPEVPCPYDYIKIKVGPKVLGPFCGEKAPEPISTQSHSVLILFHSDNSGENRGWRLSYRAAGNECPELQPPVHGKIEPSQAKYFFKDQVLVSCDTGYKVLKDNVEMDTFQIECLKDGTWSNKIPTCKIVDCRAPGELEHGLITFSTRNNLTTYKSEIKYSCQEPYYKMLNNNTGIYTCSAQGVWMNKVLGRSLPTCLPECGQPSRSLPSLVKRIIGGRNAEPGLFPWQALIVVEDTSRVPNDKWFGSGALLSASWILTAAHVLRSQRRDTTVIPVSKEHVTVYLGLHDVRDKSGAVNSSAARVVLHPDFNIQNYNHDIALVQLQEPVPLGPHVMPVCLPRLEPEGPAPHMLGLVAGWGISNPNVTVDEIISSGTRTLSDVLQYVKLPVVPHAECKTSYESRSGNYSVTENMFCAGYYEGGKDTCLGDSGGAFVIFDDLSQRWVVQGLVSWGGPEECGSKQVYGVYTKVSNYVDWVWEQMGLPQSVVEPQVERHHHHHH

Approximately 120.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 200 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Thiel S, et al. Mannan-binding lectin (MBL)-associated serine protease-1 (MASP-1), a serine proteaseassociated with humoral pattern-recognition molecules: normal and acute-phase levels in serumand stoichiometry of lectin pathway components. Clin Exp Immunol. 2012 Jul;169(1):38-48.  [Content Brief]

  [2]. Dobó J, et al. MASP-1, a promiscuous complement protease: structure of its catalytic region reveals the basisof its broad specificity. J Immunol. 2009 Jul 15;183(2):1207-14.  [Content Brief]