1. 重组蛋白
  2. Others
  3. MFG-E8 蛋白, Mouse (HEK293, His)

MFG-E8 蛋白, Mouse (HEK293, His)

目录号: HY-P77749
COA 产品使用指南

MFG-E8蛋白在促进多种组织中凋亡细胞的吞噬清除中发挥着关键作用。作为一种特异性配体,它与 alpha-v/beta-3 和 alpha-v/beta-5 受体相互作用,并且还独立地与富含磷脂酰丝氨酸的细胞表面结合。MFG-E8 蛋白, Mouse (HEK293, His) 是重组的 MFG-E8 蛋白,由 HEK293 表达,带有 N-His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥400 In-stock
10 μg ¥630 In-stock
20 μg ¥1000 In-stock
50 μg ¥2200 In-stock
100 μg ¥3750 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The MFG-E8 protein plays a critical role in promoting the phagocytic clearance of apoptotic cells in various tissues.As a specific ligand, it interacts with alpha-v/beta-3 and alpha-v/beta-5 receptors and also binds independently to phosphatidylserine-rich cell surfaces.MFG-E8 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MFG-E8 protein, expressed by HEK293 , with N-His labeled tag.

研究背景

MFG-E8(乳脂球表皮生长因子 8)是一种多功能蛋白质,对各种组织中凋亡细胞的吞噬清除有显着贡献。MFG-E8 作为 alpha-v/beta-3 和 alpha-v/beta-5 受体的特异性配体,有助于吞噬细胞识别和清除凋亡细胞。此外,它还表现出与富含磷脂酰丝氨酸的细胞表面的结合能力,与受体无关。凭借其作为透明带结合蛋白的作用,MFG-E8 可能有助于配子相互作用(通过相似性)。值得注意的是,MFG-E8 对于维持肠上皮稳态和促进粘膜愈合至关重要,凸显了其在胃肠道健康中的重要性。此外,MFG-E8 在促进 VEGF 依赖性新血管形成中发挥着关键作用,强调其参与血管生成和组织修复过程。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 35.78 ng/mL, corresponding to a specific activity is 2.795×104 units/mg.

种属

Mouse

表达系统

HEK293

标签

N-His

蛋白编号

P21956 (A23-C463)

基因 ID
蛋白结构
N-term
His
MFG-E8 (A23-C463)
Accession # P21956
C-term
同用名
Lactadherin; MFGM; MFG-E8; SED1; MP47; BA46; EDIL1; HsT19888; Medin; MFG1; OAcGD3S; SPAG10
氨基酸序列

ASGDFCDSSLCLNGGTCLTGQDNDIYCLCPEGFTGLVCNETERGPCSPNPCYNDAKCLVTLDTQRGDIFTEYICQCPVGYSGIHCETETNYYNLDGEYMFTTAVPNTAVPTPAPTPDLSNNLASRCSTQLGMEGGAIADSQISASSVYMGFMGLQRWGPELARLYRTGIVNAWTASNYDSKPWIQVNLLRKMRVSGVMTQGASRAGRAEYLKTFKVAYSLDGRKFEFIQDESGGDKEFLGNLDNNSLKVNMFNPTLEAQYIKLYPVSCHRGCTLRFELLGCELHGCSEPLGLKNNTIPDSQMSASSSYKTWNLRAFGWYPHLGRLDNQGKINAWTAQSNSAKEWLQVDLGTQRQVTGIITQGARDFGHIQYVASYKVAHSDDGVQWTVYEEQGSSKVFQGNLDNNSHKKNIFEKPFMARYVRVLPVSWHNRITLRLELLGC

分子量

Approximately 60-75 kDa due to the glycosylation.

纯度

Greater than 95% as determined by Tris-Bis PAGE

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MFG-E8 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
MFG-E8 Protein, Mouse (HEK293, His)
目录号:
HY-P77749
需求量: