MIP-5/CCL15 蛋白, Human

MIP-5/CCL15 Protein, Human is a CC chemokine with strong chemotactic properties towards myeloid cells such as dendritic cells, monocytes, neutrophils and some T-lymphocytes. MIP-5/CCL15 Protein, Human binds to chemokine receptors CCR1 and CCR3 and plays a key role in leukocyte recruitment and inflammatory disease development. development. MIP-5/CCL15 Protein, Human is a recombinant human MIP-5/CCL15 (Q22-I113) expressed by E. coli^[1].

CCL15，也称为巨噬细胞抑制蛋白 5 (MIP-5)、白细胞趋化因子 1 (Lkn-1) 和人 CC 趋化因子 2 (HCC-2)，是属于 CC 趋化因子家族的一种小细胞因子，CC 趋化因子家族是位于位于人类 17 号染色体上，具有与 CC 基序趋化因子配体 5 (CCL5) 和 CC 基序趋化因子配体 3 (CCL3) 相似的基因序列。CCL15 可在肝脏、小肠、结肠和肺的某些白细胞和巨噬细胞中表达^[1]。CCL15作为中性粒细胞、单核细胞和淋巴细胞的趋化剂，可以与趋化因子受体CCR1和CCR3结合，其中CCR3是人嗜酸性粒细胞的主要受体，在单核细胞、淋巴细胞和中性粒细胞的迁移中发挥重要作用。同时，CCL15在造血细胞调节和宿主防御中发挥效应分子作用。重组人 CCL15 也是滤泡性甲状腺癌 (FTC) 中最丰富的趋化因子。据报道，III 期结节病患者的支气管肺泡灌洗液 (BALF) 和严重持续性哮喘患者的外周血中 CCL15 升高，通过靶向其受体（特别是 CCR1），导致疾病的严重性和持续性。自分泌方式^[2][3]。

CCL15 (0.1-1000 ng/mL) can mediate human eosinophilic leukemia EoL-1 cells migration and increase PKCσ activity in a time-dependent manner via the Ptx-sensitive Gi/Go protein and PLC. Besides, it enhances butyric acid-induced EoL-1 cell differentiation^[2].
CCL15 is the most abundant chemokine in follicular thyroid carcinoma(FTC). 51.4-fold more CCL15 mRNA is present in FTC than in follicular adenoma. Condition medium of FTC cell lines induced THP-1 cell chemotaxis by 33 ~ 77%, and anti-CCL15 neutralizing antibodies reduces THP-1 cell migration in a dose-dependent manner^[3].

1. The ED₅₀ is <2 μg/mL as measured by CHO cells transfected with human CCR1, corresponding to a specific activity of >500 units/mg.
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 6.04 ng/mL, corresponding to a specific activity is 1.56×10⁵ U/mg.
3. Fuly biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human Tlymphocytes is in a concentration range of 1.0-10 ng/mL.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 6.04 ng/mL, corresponding to a specific activity is 1.56×10⁵ U/mg.

Human

E. coli

Tag Free

Q16663 (Q22-I113)

6359  [NCBI]

QFINDAETELMMSKLPLENPVVLNSFHFAADCCTSYISQSIPCSLMKSYFETSSECSKPGVIFLTKKGRQVCAKPSGPGVQDCMKKLKPYSI

Approximately 10.2 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yasuo Shimizu, et al. CC-chemokine CCL15 expression and possible implications for the pathogenesis of IgE-related severe asthma. Mediators Inflamm. 2012;2012:475253.  [Content Brief]

  [2]. Ji-Sook Lee, et al. Leukotactin-1/CCL15 induces cell migration and differentiation of human eosinophilic leukemia EoL-1 cells through PKCdelta activation. Mol Biol Rep. 2010 Jun;37(5):2149-56.  [Content Brief]

  [3]. Feng-Jiao Huang, et al. Follicular thyroid carcinoma but not adenoma recruits tumor-associated macrophages by releasing CCL15. BMC Cancer. 2016 Feb 15;16:98.  [Content Brief]