Mucin-1/MUC1 蛋白, Human (269a.a, HEK293, His)

Mucin-1/MUC1 protein and its α subunit have dual functions of adhesion and anti-adhesion proteins, forming a protective layer on epithelial cells. At the same time, the β subunit and its C-terminal domain participate in cell signaling through phosphorylation and protein-protein interactions. Mucin-1/MUC1 Protein, Human (269a.a, HEK293, His) is the recombinant human-derived Mucin-1/MUC1 protein, expressed by HEK293 , with C-His labeled tag.

Mucin-1/MUC1 蛋白表现出多种功能作用：α 亚基具有细胞粘附特性，并作为粘附蛋白和抗粘附蛋白发挥作用，可能在上皮细胞上形成一层保护层，抵御细菌和酶的攻击。同时，β 亚基及其 C 末端结构域通过磷酸化和蛋白质-蛋白质相互作用参与细胞信号传导。Mucin-1/MUC1 调节 ERK、SRC 和 NF-kappa-B 通路中的信号传导，影响活化 T 细胞中的 Ras/MAPK 通路。此外，它在促进肿瘤进展、调节 TP53 介导的转录以及决定基因毒性应激反应中的细胞命运方面发挥着作用。值得注意的是，Mucin-1/MUC1 与 KLF4 结合，与 TP53 的 PE21 启动子元件结合，从而抑制 TP53 活性并促进复杂的细胞功能网络。

Measured by its binding ability in a functional ELISA. When Recombinant Human MUC1 is present at 1 μg/mL can bind Anti-Mucin-1 Antibody. The ED₅₀ for this effect is 5.70 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human MUC1 is present at 1 μg/mL can bind Anti-Mucin-1 Antibody. The ED₅₀ for this effect is 5.70 ng/mL.

Human

HEK293

C-His

P15941-1 (S890-G1158)

4582  [NCBI]

STAPPAHGVTSAPDTRPAPGSTAPPAHGVTSAPDTRPAPGSTAPPAHGVTSAPDNRPALGSTAPPVHNVTSASGSASGSASTLVHNGTSARATTTPASKSTPFSIPSHHSDTPTTLASHSTKTDASSTHHSSVPPLTSSNHSTSPQLSTGVSFFFLSFHISNLQFNSSLEDPSTDYYQELQRDISEMFLQIYKQGGFLGLSNIKFRPGSVVVQLTLAFREGTINVHDVETQFNQYKTEAASRYNLTISDVSVSDVPFPFSAQSGAGVPG

42-95 kDa and 8-10 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.