Nectin-1 蛋白, Mouse (HEK293, His)

Nectin-1 is critical in cell adhesion and synaptogenesis, promotes neurite outgrowth, and serves as a receptor for alphaherpesviruses, including HSV-1, HSV-2, and pseudorabies virus. It promotes viral entry and is involved in homodimerization, trans-heterodimerization with NECTIN3, and interactions with FGFR, AFDN, and HSV glycoprotein D. Nectin-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Nectin-1 Protein, Mouse (HEK293, His) is 324 a.a., with molecular weight of ~37.6 KDa.

Nectin-1 是细胞粘附和突触发生的关键参与者，具有神经突生长促进活性，强调了其在神经发育过程中的重要性。Nectin-1 作为 α 疱疹病毒（包括 HSV-1、HSV-2 和伪狂犬病病毒）的受体，可促进病毒进入细胞。该蛋白参与顺式和反式同二聚化，还可以与 NECTIN3 形成反式异二聚体，启动对轴突-树突相互作用至关重要的点粘附连接的形成。此外，它与 FGFR1、FGFR2 和 FGFR3 以及 AFDN 的相互作用，与肌动蛋白细胞骨架建立了联系，有助于其发挥多种细胞作用。值得注意的是，Nectin-1 与 HSV 糖蛋白 D (gD) 相互作用，强调了它参与病毒感染过程。

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1. The ED₅₀ for this effect is 151 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1.The ED₅₀ for this effect is 151 ng/mL.

Mouse

HEK293

C-His

Q9JKF6 (Q31-A354)

58235  [NCBI]

QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKASNGSKQNMAIYNPTMGVSVLPPYEKRVEFLRPSFIDGTIRLSGLELEDEGMYICEFATFPTGNRESQLNLTVMAKPTNWIEGTRAVLRARKGQDDKVLVATCTSANGKPPSAVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHRQSLACIVNYHLDRFRESLTLNVQYEPEVTIEGFDGNWYLQRTDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFRGPITYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPTPEHGRRAGQMPTA

Approximately 50-75 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.