NEDD8 蛋白, Human

NEDD8 is an important ubiquitin-like protein that plays a key role in cell cycle regulation and embryogenesis by binding to specific proteins such as cullin and p53/TP53. Its binding to cullin is critical for recruiting E2 enzymes to the cullin-RING-based E3 ubiquitin-protein ligase complex, thereby enabling degradation of regulatory proteins. NEDD8 Protein, Human is the recombinant human-derived NEDD8 protein, expressed by E. coli , with tag free. The total length of NEDD8 Protein, Human is 76 a.a., with molecular weight of ~9.0 kDa.

NEDD8 是一种类泛素蛋白，通过与一组选定的细胞蛋白（包括 cullin 和 p53/TP53）结合，在调节细胞周期进程和胚胎发生中发挥关键作用。NEDD8 与 cullin 的连接对于将 E2 酶招募到基于 cullin-RING 的 E3 泛素-蛋白质连接酶复合物至关重要，从而促进调节蛋白（如细胞周期蛋白）的多泛素化和随后的蛋白酶体降解。对于 p53/TP53，NEDD8 缀合会抑制其转录活性。这种共价附着过程依赖于 E1 复合物 UBE1C-APPBP1 的预先激活以及与 E2 酶 UBE2M 的连接。NEDD8 还与 AHR 和 NUB1 直接互动，进一步扩大其监管网络。

Human

E. coli

Tag Free

Q15843 (M1-G76)

4738  [NCBI]

MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG

Approximately 9.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.