Netrin receptor DCC/DCC 蛋白, Human (HEK293, His)

The netrin receptor DCC guides axonal attraction in neuronal growth cones during nervous system development. Interaction with UNC5 protein induces axonal repulsion. Netrin receptor DCC/DCC Protein, Human (HEK293, His) is the recombinant human-derived Netrin receptor DCC/DCC protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of Netrin receptor DCC/DCC Protein, Human (HEK293, His) is 1066 a.a., with molecular weight of 125-150 kDa.

DCC 是 netrin 的关键受体，在神经系统发育过程中配体结合时介导轴突对神经元生长锥的吸引，从而在轴突引导中发挥关键作用。它与 UNC5 蛋白的复杂关联可能会触发导致轴突排斥的信号通路。此外，DCC 作为依赖性受体，在不与 netrin 配体结合时引发细胞凋亡。除了在神经发育中的作用之外，DCC 还被认为是一种肿瘤抑制基因。该受体参与多种蛋白质相互作用，包括 UNC5A、UNC5B、UNC5C 和可能的 UNC5D 的细胞质部分，以及与 DSCAM、PTK2/FAK1、MAPK1、NTN1、MYO10、CBLN4、SIAH1 和 SIAH2 的相互作用。这些相互作用强调了 DCC 在协调轴突引导、细胞凋亡和潜在肿瘤抑制方面的多方面功能。

Immobilized Recombinant Human DCC at 1 μg/ml (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED₅₀ for this effect is 85.29 ng/mL.

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  Immobilized Recombinant Human DCC at 1 μg/ml (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED₅₀ for this effect is 85.29 ng/mL.

Human

HEK293

C-His;C-10*His

P43146-1 (F32-N1097)

1630  [NCBI]

FQIKAFTALRFLSEPSDAVTMRGGNVLLDCSAESDRGVPVIKWKKDGIHLALGMDERKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPLRFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGMYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECTVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQTSAQLIVPKPAIPSSSVLPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLQAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYSLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSVPDLSTPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSN

125-150 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.