Neurolysin 蛋白, Human (His)

Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag. The total length of Neurolysin Protein, Human (His) is 667 a.a., with molecular weight of ~67 kDa.

Neurolysin 蛋白是一种多功能酶，能够水解各种寡肽，包括神经降压素、缓激肽和强啡肽 A。除了在寡肽代谢中的作用外，Neurolysin 还在大麻素信号通路中发挥调节功能。它通过促进加压素（一种与大麻素受体 CNR1 相互作用的拮抗肽）的降解来实现这一目标。这种双重作用使 Neurolysin 在生物活性肽的酶促分解和通过调节血加压素水平调节大麻素受体信号传导方面发挥着关键作用。

Measured by its ability to cleave a fluorogenic peptide substrate (7-methoxycoumarin-4-yl) acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH and the specific activity is > 100 pmol/min/μg.

Human

E. coli

N-His

Q9BYT8 (S38-P704)

57486  [NCBI]

SSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Approximately 67 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.