Nicastrin 蛋白, Human (HEK293, His)

Nicastrin Protein, a crucial gamma-secretase complex subunit, catalyzes intramembrane cleavage of Notch receptors and APP.Operating in Notch and Wnt signaling, the complex modulates regulatory proteins and CTNNB1 levels.Collaborating with PSEN1/2, APH1A/B, and PSENEN/PEN2, Nicastrin binds to proteolytically processed C83 and C99 fragments, emphasizing its pivotal role in molecular pathways and cellular processes.Nicastrin Protein, Human (HEK293, His) is the recombinant human-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag.

Nicastrin 蛋白是 γ-分泌酶复合物的重要亚基，γ-分泌酶复合物是一种内切蛋白酶组装体，对于催化整合膜蛋白（包括 Notch 受体和淀粉样蛋白-β 前体蛋白 (APP)）的膜内裂解至关重要。γ-分泌酶复合物在 Notch 和 Wnt 信号级联中运行，通过处理关键调节蛋白和调节胞质 CTNNB1 水平来调节下游过程。作为 γ-分泌酶复合物的基本成分，尼卡斯特林与早老素同型二聚体（PSEN1 或 PSEN2）、APH1（APH1A 或 APH1B）和 PSENEN/PEN2 协同形成功能复合物。这种复杂的分子机制与 APP 的蛋白水解加工的 C 末端片段 C83 和 C99 结合，并与 PSEN1 和 PSEN2 相互作用，强调了其在分子途径和细胞过程中的重要作用。

Human

HEK293

C-His

Q92542-1 (N34-E669)

23385  [NCBI]

NSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELE

Approximately 105-125 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.