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Nicastrin 蛋白, Human (HEK293, His)

目录号: HY-P74698
COA 产品使用指南

Nicastrin 蛋白是一种重要的 γ 分泌酶复合物亚基,可催化 Notch 受体和 APP 的膜内裂解。该复合物在 NotchWnt 信号传导中发挥作用,调节调节蛋白和 CTNNB1 水平。Nicastrin 与 PSEN1/2、APH1A/B 和 PSENEN/PEN2 合作,与蛋白水解加工的 C83 和 C99 片段结合,强调其在分子途径和细胞过程中的关键作用。Nicastrin 蛋白, Human (HEK293, His) 是重组的 烟碱蛋白,由 HEK293 表达,带有 C-His 标签。

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10 μg ¥1350 In-stock
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100 μg ¥6500 In-stock
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描述

Nicastrin Protein, a crucial gamma-secretase complex subunit, catalyzes intramembrane cleavage of Notch receptors and APP.Operating in Notch and Wnt signaling, the complex modulates regulatory proteins and CTNNB1 levels.Collaborating with PSEN1/2, APH1A/B, and PSENEN/PEN2, Nicastrin binds to proteolytically processed C83 and C99 fragments, emphasizing its pivotal role in molecular pathways and cellular processes.Nicastrin Protein, Human (HEK293, His) is the recombinant human-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag.

研究背景

Nicastrin 蛋白是 γ-分泌酶复合物的重要亚基,γ-分泌酶复合物是一种内切蛋白酶组装体,对于催化整合膜蛋白(包括 Notch 受体和淀粉样蛋白-β 前体蛋白 (APP))的膜内裂解至关重要。γ-分泌酶复合物在 NotchWnt 信号级联中运行,通过处理关键调节蛋白和调节胞质 CTNNB1 水平来调节下游过程。作为 γ-分泌酶复合物的基本成分,尼卡斯特林与早老素同型二聚体(PSEN1 或 PSEN2)、APH1(APH1A 或 APH1B)和 PSENEN/PEN2 协同形成功能复合物。这种复杂的分子机制与 APP 的蛋白水解加工的 C 末端片段 C83 和 C99 结合,并与 PSEN1 和 PSEN2 相互作用,强调了其在分子途径和细胞过程中的重要作用。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q92542-1 (N34-E669)

基因 ID
蛋白结构
N-term
Nicastrin (N34-E669)
Accession # Q92542
His
C-term
同用名
Nicastrin; NCSTN; KIAA0253
氨基酸序列

NSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELE

分子量

Approximately 105-125 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Nicastrin Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Nicastrin Protein, Human (HEK293, His)
目录号:
HY-P74698
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