Noggin 蛋白, Mouse (CHO)

Noggin Protein, Mouse (CHO) is a bone morphogenetic protein (BMP) antagonist.

哺乳动物头蛋白的序列与非洲爪蟾头蛋白非常相似，并且在非洲爪蟾胚胎组织上进行的诱导测定中具有相似的活性。在成年哺乳动物中，头蛋白在神经系统中的表达最为显着^[1]。Noggin 是一种骨形态发生蛋白 (BMP) 拮抗剂，在 Spemann 组织中表达。鼠Noggin 在浓缩软骨和未成熟软骨细胞中表达。在缺乏 Noggin 的小鼠中，软骨凝结正常启动，但出现增生，关节发育启动失败^[2]。Noggin 蛋白以高亲和力结合 BMP4，并可通过阻断与同源细胞表面受体的结合来消除 BMP4 活性^[3]。人头蛋白 (hNoggin) 在去除其信号序列（残基 1-27）后有 205 个氨基酸（残基 28-232），并作为糖基化、共价连接的同二聚体分泌。人 Noggin 与 hBMP-4 具有高亲和力（而与 hBMP-7^[4] 具有较低亲和力。

1.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is ≤0.01 μg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (HY-P7007), corresponding to a specific activity is ≥1×10⁵units/mg.
2.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is ≤0.06 μg/mL in the presence of 10 ng/mL of Recombinant Human BMP-4 (HY-P7007), corresponding to a specific activity is ≥1.7×10⁷units/mg.
3.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.0571 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (HY-P7007), corresponding to a specific activity is 1.751×10⁴ units/mg.

• 
  Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.0868 μg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4, corresponding to a specific activity is 1.152×10⁴ units/mg.

Mouse

CHO

Tag Free

P97466 (L20-C232)

18121  [NCBI]

LRAAPAGGQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

Approximately 28-31 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM PB, 500 mM NaCl , pH 7.4, 5% trehalose, mannitol and 0.01% Tween 80.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Valenzuela DM, et al. Identification of mammalian noggin and its expression in the adult nervous system. J Neurosci. 1995 Sep;15(9):6077-84.  [Content Brief]

  [2]. Brunet LJ, et al. Noggin, cartilage morphogenesis, and joint formation in the mammalian skeleton. Science. 1998 May 29;280(5368):1455-7.  [Content Brief]

  [3]. Smith WC, et al. Secreted noggin protein mimics the Spemann organizer in dorsalizing Xenopus mesoderm. Nature. 1993 Feb 11;361(6412):547-9.  [Content Brief]

  [4]. Groppe J, et al. Structural basis of BMP signalling inhibition by the cystine knot protein Noggin. Nature. 2002 Dec 12;420(6916):636-42.  [Content Brief]