Nogo Receptor/NgR 蛋白, Mouse (HEK293, His)

Nogo receptor/NgR proteins act as receptors for RTN4, OMG, MAG, and sialylated gangliosides. It also binds chondroitin sulfate proteoglycans and heparin. Nogo Receptor/NgR Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nogo Receptor/NgR protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Nogo Receptor/NgR Protein, Mouse (HEK293, His) is 421 a.a., with molecular weight of 75-85 kDa.

Nogo 受体/NgR 蛋白充当 RTN4、OMG 和 MAG 以及唾液酸化神经节苷脂 GT1b 和 GM1 的受体。此外，它还充当硫酸软骨素蛋白聚糖的受体，并可以结合肝素。细胞内信号级联通过辅助受体 NGFR 启动，导致 Rho 激活以及随后肌动蛋白细胞骨架的重组。这种信号机制介导轴突生长抑制，并在调节成人中枢神经系统内的轴突再生和神经元可塑性方面发挥着至关重要的作用。此外，Nogo受体/NgR参与出生后大脑发育，在轴突穿过大脑中线的迁移和胼胝体的形成中发挥必要的作用。它还可能通过与 MAG 相互作用，防止运动神经元凋亡。它与 RTN4 和 LINGO1 结合使用，调节皮质发育过程中的神经元前体细胞运动。与其他家族成员一样，Nogo 受体/NgR 有助于限制大脑发育过程中形成的树突棘和突触的数量。它形成同二聚体并与各种蛋白质相互作用，包括 MAG、RTN4、NGFR、LINGO1 和 OLFM1，突出了其在细胞信号传导和神经发育中的多方面作用。

Mouse

HEK293

C-6*His

Q99PI8 (C27-S447)

65079  [NCBI]

CPGACVCYNEPKVTTSCPQQGLQAVPTGIPASSQRIFLHGNRISHVPAASFQSCRNLTILWLHSNALARIDAAAFTGLTLLEQLDLSDNAQLHVVDPTTFHGLGHLHTLHLDRCGLRELGPGLFRGLAALQYLYLQDNNLQALPDNTFRDLGNLTHLFLHGNRIPSVPEHAFRGLHSLDRLLLHQNHVARVHPHAFRDLGRLMTLYLFANNLSMLPAEVLMPLRSLQYLRLNDNPWVCDCRARPLWAWLQKFRGSSSEVPCNLPQRLADRDLKRLAASDLEGCAVASGPFRPIQTSQLTDEELLSLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDTPPGNGSGPRHINDSPFGTLPSSAEPPLTALRPGGSEPPGLPTTGPRRRPGCSRKNRTRSHCRLGQAGSGASGTGDAEGS

75-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.