NOTCH1 蛋白, Mouse (sf9, His)

NOTCH1 Protein acts as a receptor for membrane-bound ligands (JAG1, JAG2, and DLL1), determining cell fate.Upon activation, NICD forms a transcriptional activator complex, influencing differentiation, proliferation, and apoptosis.NOTCH1 negatively regulates angiogenesis, endothelial cell behavior, and impacts various developmental processes.It interacts with multiple partners, illustrating its involvement in signaling networks.NOTCH1 Protein, Mouse (sf9, His) is the recombinant mouse-derived NOTCH1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

NOTCH1 蛋白作为膜结合配体的受体，包括 Jagged-1 (JAG1)、Jagged-2 (JAG2) 和 Delta-1 (DLL1)，在细胞命运决定中发挥着至关重要的作用。配体激活后，释放的Notch胞内结构域（NICD）与RBPJ/RBPSUH形成转录激活剂复合物，激活分裂位点增强子中的基因并影响分化、增殖和凋亡。NOTCH1 参与血管生成，负向调节内皮细胞增殖、迁移和血管生成萌芽。它有助于 CD4+ 和 CD8+ 细胞的胸腺成熟、滤泡分化以及滤泡内可能的细胞命运选择。在小脑发育中，它作为神经元 DNER 的受体，影响伯格曼胶质细胞的分化。NOTCH1 还在抑制神经元和肌原性分化、植入后发育、中胚层发育、体节形成和神经发生中发挥作用。此外，它还调节活动纤毛和不动纤毛之间的平衡，以确定左/右对称性。该蛋白与各种伙伴相互作用，包括 DNER、DTX1、DTX2、RBPJ/RBPSUH、MAML1、MAML2、MAML3、SNW1、AAK1、FBXW7、SGK1、HIF1AN、SNAI1、MDM2A、BCL6、THBS4、CCN3、DLL4、ZMIZ1、MEGF10、 DLL1、JAG1、PRAG1、PSEN1 和 ZFP64，强调其参与复杂的信号网络和调控过程。

Immobilized NOTCH1, Mouse (sf9, His) at 2 μg/mL (100 μL/well) can bind mouse DLL4-mFc, The EC₅₀ of NOTCH1, Mouse (sf9, His) is ≤0.2 μg/mL.

Mouse

Sf9 insect cells

C-His

Q01705 (A19-Q526)

18128  [NCBI]

ARGLRCSQPSGTCLNGGRCEVANGTEACVCSGAFVGQRCQDSNPCLSTPCKNAGTCHVVDHGGTVDYACSCPLGFSGPLCLTPLDNACLANPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFESSYICRCPPGFHGPTCRQDVNECSQNPGLCRHGGTCHNEIGSYRCACRATHTGPHCELPYVPCSPSPCQNGGTCRPTGDTTHECACLPGFAGQNCEENVDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFQGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECALGANPCEHAGKCLNTLGSFECQCLQGYTGPRCEIDVNECISNPCQNDATCLDQIGEFQCICMPGYEGVYCEINTDECASSPCLHNGHCMDKINEFQCQCPKGFNGHLCQ

Approximately 80 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.