Notum 蛋白, Human (C330S, CHO, His)

Notum Protein serves as a crucial negative regulator of the Wnt signaling pathway, playing a specific role in mediating the depalmitoleoylation of WNT proteins. The process of serine palmitoleoylation of WNT proteins is essential for their efficient binding to frizzled receptors, and Notum's carboxylesterase activity is instrumental in modulating this aspect of the Wnt signaling cascade. Notum Protein, Human (C330S, CHO, His) is the recombinant human-derived Notum protein, expressed by CHO , with C-His labeled tag. The total length of Notum Protein, Human (C330S, CHO, His) is 371 a.a., with molecular weight of 36-45 kDa.

Notum 是一种羧酸酯酶，作为 Wnt 信号通路的关键负调节因子发挥作用。该酶在介导 WNT 蛋白的去棕榈油酰化中发挥特定作用。WNT 蛋白的丝氨酸棕榈酰化对于其与卷曲受体的有效结合至关重要，这是 Wnt 信号传导激活的关键步骤。通过催化从 WNT 蛋白中去除棕榈油酸酯部分，Notum 减弱了 Wnt 信号传导，有助于对该途径的微调。它必须强调 Notum 通过靶向 WNT 蛋白的脂质修饰来调节 Wnt 信号传导的重要性，强调其作为这一重要细胞途径中关键调节因子的作用。

Measured by its esterase activity. The specific activity is 1118.156 pmol/min/µg, as measured under the described conditions.

Human

CHO

C-6*His

Q6P988 (S81-T451, C330S)

147111  [NCBI]

SAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESRGSRRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRSPVFVVQWLFDEAQLTVDNVHLTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHDSHKASKTPLKGCPVHLVDSCPWPHCNPSCPT

Approximately 36-45 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.