NUDT2 蛋白, Human (His)

The NUDT2 protein has a dual enzymatic role as a catalyst for the asymmetric hydrolysis of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to generate AMP and ATP. This activity highlights its involvement in nucleotide metabolism and the regulation of cellular purine nucleotide pools. NUDT2 Protein, Human (His) is the recombinant human-derived NUDT2 protein, expressed by E. coli , with N-His labeled tag. The total length of NUDT2 Protein, Human (His) is 147 a.a., with molecular weight of ~18.3 kDa.

NUDT2（或 nudix 水解酶 2）是一种在核苷酸代谢中发挥关键作用的酶。它催化二腺苷 5',5'''-P1,P4-四磷酸 (Ap4A) 的不对称水解，从而产生 AMP 和 ATP。这种酶活性有助于细胞核苷酸库的调节。此外，NUDT2 在体外表现出脱帽活性，靶向 FAD 加帽的 RNA 和 dpCoA 加帽的 RNA。这种双重功能表明它参与了 RNA 周转过程。参考文献强调了 NUDT2 在核苷酸代谢中的重要性，强调了其水解 Ap4A 的能力及其在 RNA 脱帽活动中的潜在作用，有助于更广泛地了解其在与核苷酸稳态和 RNA 代谢相关的细胞过程中的功能。

Measured by its ability to the proliferation of MCF-7 human breast cancer cells. The ED₅₀ for this effect is 3.005 μg/mL, corresponding to a specific activity is 332.7787 Unit/mg.

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  Measured by its ability to the proliferation of MCF-7 human breast cancer cells. The ED₅₀ for this effect is 3.005 μg/mL, corresponding to a specific activity is 332.7787 Unit/mg.

Human

E. coli

N-His

P50583 (M1-A147)

318  [NCBI]

MALRACGLIIFRRCLIPKVDNNAIEFLLLQASDGIHHWTPPKGHVEPGEDDLETALRETQEEAGIEAGQLTIIEGFKRELNYVARNKPKTVIYWLAEVKDYDVEIRLSHEHQAYRWLGLEEACQLAQFKEMKAALQEGHQFLCSIEA

Approximately 18.3-19 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.