OSM 蛋白, Human (227a.a)

OSM Protein, Human (227a.a) is a polypeptide chain containing 227 amino acids. Oncostatin M is a cytokine belonging to the IL-6 family that has multiple functions in hematopoiesis, mesenchymal stem cell differentiation, liver regeneration, heart remodeling, nociception, inflammation and metabolism.

制瘤素M是一种属于IL-6家族的细胞因子，在造血、间充质干细胞分化、肝再生、心脏重塑、伤害感受、炎症和代谢等方面具有多种功能。全长制瘤素 M 蛋白含有 239 至 263 个氨基酸，可折叠成长链四螺旋束蛋白，具有代表所有其他 IL-6 家族细胞因子的上下拓扑结构。人制瘤素-M可以结合由gp130和LIFR组成的I型受体复合物（gp130/LIFRb）或由gp130和OSMR组成的II型受体复合物（gp130/OSMRb）。在小鼠系统中，制瘤素-M 已被证明仅以高亲和力结合 II 型 gp130/OSMRb 复合物^[1][2]。制瘤素-M 与特定受体复合物结合，然后激活两条主要信号传导途径：Janus 激酶信号转导器和转录激活剂 (JAK-STAT) 以及丝裂原激活蛋白激酶 (MAPK)，以调节下游事件^[3] 。

The ED₅₀ is <10 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of >1.0 × 10⁵ units/mg.

Human

E. coli

Tag Free

P13725 (A26-R252)

5008  [NCBI]

AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRRTRPSRKGKRLMTRGQLPR

Approximately 25.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hermanns HM, et al. Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. Cytokine Growth Factor Rev. 2015 Oct;26(5):545-58.  [Content Brief]

  [2]. Gómez-Lechón MJ, et al. Oncostatin M: signal transduction and biological activity. Life Sci. 1999;65(20):2019-30.  [Content Brief]

  [3]. Chen SH, et al. Oncostatin M: a pleiotropic cytokine in the central nervous system. Cytokine Growth Factor Rev. 2004 Oct;15(5):379-91.  [Content Brief]