Osteopontin/OPN 蛋白, Mouse (P.pastoris, His)

Osteopontin/OPN Protein, a crucial non-collagenous bone protein, binds strongly to hydroxyapatite, integral to the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Moreover, as a cytokine, OPN efficiently induces interferon-gamma and interleukin-12 production while inhibiting interleukin-10, pivotal in promoting type I immunity.Osteopontin/OPN Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Osteopontin/OPN protein, expressed by P.pastoris , with N-6*His labeled tag.

骨桥蛋白 (OPN) 是一种重要的非胶原骨蛋白，对羟基磷灰石具有很强的亲和力，是矿化基质的组成部分。它可能在促进细胞和细胞外基质之间的相互作用方面发挥着至关重要的作用。此外，OPN 作为细胞因子，有效刺激干扰素 γ 和白细胞介素 12 的产生，同时抑制白细胞介素 10 的产生。这些功能对于促进 I 型免疫的途径至关重要。

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 ℃. The ED₅₀ for this effect is 0.5101 μg/mL, corresponding to a specific activity is 1.960×10⁴ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.5101 μg/mL, corresponding to a specific activity is 1.960×10⁴ units/mg.

Mouse

P. pastoris

N-6*His

P10923 (L17-S290)

20750  [NCBI]

LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSS

Approximately 60 kDa in reducing SDS-PAGE due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.