OTUB2 蛋白, Human (His)

OTUB2 is a hydrolase that efficiently removes conjugated ubiquitin from proteins in vitro, implying a crucial role in preventing protein degradation and regulating turnover. Its deubiquitinating activity targets “Lys-11”, “Lys-48” and “Lys-63” linked polyubiquitin chains, with preference for the latter. OTUB2 Protein, Human (His) is the recombinant human-derived OTUB2 protein, expressed by E. coli , with N-6*His labeled tag.

作为一种水解酶，OTUB2 表现出在体外有效去除蛋白质中缀合泛素的能力，这表明通过防止降解在蛋白质周转中发挥着关键的调节作用。凭借其去泛素化活性，OTUB2 作用于“Lys-11”、“Lys-48”和“Lys-63”连接的多聚泛素链，显示出对后者的偏好。这种特异性强调了 OTUB2 在调节多种泛素连接方面的选择性，强调其在协调与蛋白质泛素化和降解相关的细胞过程中的潜在意义。

The specific activity was determined to be 73.477 nmol/min/mg in a DUB assay.

Human

E. coli

N-6*His

Q96DC9-1 (M1-H234)

78990  [NCBI]

MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH

Approximately 28 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 500 mM arginine, pH 10.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.