P-selectin 蛋白, Human (HEK293, His)

P-selectin is a Ca(2+)-dependent receptor on myeloid cells that critically binds to sialic acid-Lewis X on neutrophils and monocytes, promoting the interaction between activated endothelial cells or platelets and leukocytes interaction. This binding primarily to SELPLG/PSGL1 and PODXL2 is critical for rapid rolling of leukocytes during early inflammation. P-selectin Protein, Human (HEK293, His) is the recombinant human-derived P-selectin protein, expressed by HEK293 , with C-His labeled tag. The total length of P-selectin Protein, Human (HEK293, His) is 730 a.a., with molecular weight of 95-130 kDa.

P-选择素是骨髓细胞上的一种 Ca(2+) 依赖性受体，有助于与中性粒细胞和单核细胞上的碳水化合物结合，在活化的内皮细胞或血小板与白细胞的相互作用中发挥着至关重要的作用。P-选择素识别的配体是唾液酸-Lewis X，这种相互作用对于炎症初始阶段白细胞在血管表面快速滚动至关重要，这是通过与 SELPLG 的结合来实现的。此外，P-选择素与 SNX17 形成相互作用，并通过与 SELPLG/PSGL1 和 PODXL2 的结合介导中性粒细胞的粘附和滚动。这种相互作用取决于 SELPLG 和 PODXL2 的唾液酸-Lewis X 表位，以及 SELPLG 上的特定酪氨酸硫酸化。

1.Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ for this effect is 14.18 μg/mL, corresponding to a specific activity is 70.5219 units/mg.
2.Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin, immobilized at 10 µg/mL, will induce ≥72.39% adhesion on U937 cells (100 µL/well at 1 x 10⁶cells/mL).

• 
  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.The ED₅₀ for this effect is 14.18 μg/mL, corresponding to a specific activity is 70.5219 units/mg.

Human

HEK293

C-His

P16109 (W42-A771)

6403  [NCBI]

WTYHYSTKAYSWNISRKYCQNRYTDLVAIQNKNEIDYLNKVLPYYSSYYWIGIRKNNKTWTWVGTKKALTNEAENWADNEPNNKRNNEDCVEIYIKSPSAPGKWNDEHCLKKKHALCYTASCQDMSCSKQGECLETIGNYTCSCYPGFYGPECEYVRECGELELPQHVLMNCSHPLGNFSFNSQCSFHCTDGYQVNGPSKLECLASGIWTNKPPQCLAAQCPPLKIPERGNMTCLHSAKAFQHQSSCSFSCEEGFALVGPEVVQCTASGVWTAPAPVCKAVQCQHLEAPSEGTMDCVHPLTAFAYGSSCKFECQPGYRVRGLDMLRCIDSGHWSAPLPTCEAISCEPLESPVHGSMDCSPSLRAFQYDTNCSFRCAEGFMLRGADIVRCDNLGQWTAPAPVCQALQCQDLPVPNEARVNCSHPFGAFRYQSVCSFTCNEGLLLVGASVLQCLATGNWNSVPPECQAIPCTPLLSPQNGTMTCVQPLGSSSYKSTCQFICDEGYSLSGPERLDCTRSGRWTDSPPMCEAIKCPELFAPEQGSLDCSDTRGEFNVGSTCHFSCDNGFKLEGPNNVECTTSGRWSATPPTCKGIASLPTPGLQCPALTTPGQGTMYCRHHPGTFGFNTTCYFGCNAGFTLIGDSTLSCRPSGQWTAVTPACRAVKCSELHVNKPIAMNCSNLWGNFSYGSICSFHCLEGQLLNGSAQTACQENGHWSTTVPTCQAGPLTIQEA

Approximately 95-130 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.