PCSK9 蛋白, Rhesus Macaque (HEK293, His)

PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER and LRP8/APOER2 receptors. It promotes LDLR degradation through non-proteolytic mechanisms, disrupts LDLR recycling, induces LDLR ubiquitination, and inhibits APOB degradation. PCSK9 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PCSK9 protein, expressed by HEK293 , with C-His labeled tag. The total length of PCSK9 Protein, Rhesus Macaque (HEK293, His) is 662 a.a., with molecular weight of ~20 & 62 kDa, respectively.

PCSK9蛋白在维持血液中胆固醇水平的平衡中起着至关重要的作用。它与参与脂质代谢的多种受体结合，包括 LDLR、VLDLR、LRP1/APOER 和 LRP8/APOER2，并促进它们在细胞内酸性区室中的降解。它通过网格蛋白 LDLRAP1/ARH 途径介导的非蛋白水解机制增强肝脏 LDLR 的降解。此外，它可能会破坏 LDLR 的回收，将其引导至溶酶体进行降解。PCSK9 还可以诱导 LDLR 泛素化，导致其随后崩溃。此外，它通过自噬体/溶酶体途径抑制 APOB（脂蛋白的重要组成部分）的降解，与 LDLR 无关。在早期分泌途径中，PCSK9 有助于处理 BACE1 的非乙酰化中间体。它还通过增加蛋白酶体降解来减少其表面表达，从而在调节钠离子通过 ENaC 通道的吸收方面发挥作用。最后，PCSK9 通过调节 LRP8/APOER2 的水平和相关的抗凋亡信号通路来调节神经元凋亡。

Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED₅₀ for this effect is 224.2 ng/mL

• 
  Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED₅₀ for this effect is 224.2 ng/mL.

Rhesus Macaque

HEK293

C-His

A8T666 (Q31-Q692)

717147  [NCBI]

QEDEDGDYEELVLALRSEEDGLADAPEHGATATFHRCAKDPWRLPGTYVVVLKEETHRSQSERTARRLQAQAARRGYLTKILHVFHHLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPARYRADEYQPPKGGSLVEVYLLDTSIQSDHREIEGRVMVTDFESVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGAGLRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVFNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSRSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHRAGWQLFCRTVWSAHSGPTRMATAVARCAQDEELLSCSSFSRSGKRRGERIEAQGGKRVCRAHNAFGGEGVYAIARCCLLPQVNCSVHTAPPAGASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVIVACEDGWTLTGCSPLPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSKEAVAAVAICCRSRHLVQASQELQ

Approximately 20&62 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.