PD-1 蛋白, Mouse (HEK293, His-Fc)

PD-1 (programmed cell death 1) protein negatively regulates immune responses and affects processes such as apoptosis and tolerance induction. It is a transmembrane protein found on the outside of the plasma membrane and expressed in the retina. PD-1 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived PD-1 protein, expressed by HEK293 , with C-hFc, C-8*His labeled tag.

PD-1（程序性细胞死亡1）蛋白在免疫反应的负向调节中发挥着至关重要的作用，作用于细胞凋亡过程的负向调节、耐受诱导的负向调节和细胞凋亡过程的正向调节等过程的上游。这种跨膜蛋白位于质膜的外侧，并在视网膜中表达。PD-1与多种疾病相关，包括自身免疫性疾病、乙型和丙型肝炎、肝细胞癌和狼疮性肾炎。PD-1 在成人胸腺和卵巢中的偏向表达表明其参与这些组织内的免疫调节。

1. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625 ng/mL of bound hPD-L1. The ED₅₀ for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×10³ units/mg.
2. Immobilized Recombinant Mouse PD-1 at 1 μg/ml (100 μl/well) can bind Recombinant Mouse PD-L1. The ED50 for this effect is 0.5001 μg/mL.

• 
  Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625ng/mL of bound hPD-L1. The ED₅₀ for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×10³ units/mg.

Mouse

HEK293

C-hFc;C-8*His

NP_032824.1 (L25-Q167)

18566  [NCBI]

LEVPNGPWRSLTFYPAWLTVSEGANATFTCSLSNWSEDLMLNWNRLSPSNQTEKQAAFCNGLSQPVQDARFQIIQLPNRHDFHMNILDTRRNDSGIYLCGAISLHPKAKIEESPGAELVVTERILETSTRYPSPSPKPEGRFQ

60-65 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.