PDGF-AA 蛋白, Rat (P.pastoris, His)

PDGF-AA protein is a key growth factor that serves as an effective mitogen for mesenchymal cells, coordinates embryonic development, and affects cell proliferation, migration, survival, and chemotaxis. It plays an important role in alveolar septal formation, gastrointestinal tract development, interstitial cell maturation, spermatogenesis, oligodendrocyte development, and myelination of the spinal cord and cerebellum. PDGF-AA Protein, Rat (P.pastoris, His) is the recombinant rat-derived PDGF-AA protein, expressed by P. pastoris , with N-His labeled tag.

PDGF-AA 蛋白作为关键生长因子出现，协调胚胎发育的关键方面，包括细胞增殖、迁移、存活和趋化性。PDGF-AA 因其作为间充质细胞有丝分裂原的效力而被认为在各种生理过程中发挥着重要作用，例如肺泡间隔的形成、胃肠道的发育、间质细胞成熟、精子发生、少突胶质细胞发育和髓鞘形成。脊髓和小脑。此外，PDGF-AA 是伤口愈合动力学的重要贡献者。其复杂的信号传导涉及通过与 PDGFB 形成异二聚体进行调节，强调了其调节机制的多功能性。在结构上，PDGF-AA 采用同型二聚体构型，其特征是反向平行的二硫键连接的二聚体。此外，它与PDGFB形成异二聚体，与PDGFRA同二聚体以及PDGFRA和PDGFRB形成的异二聚体相互作用，显示出其分子相互作用的复杂性。此外，PDGF-AA 表现出与 CSPG4 的相互作用，为其多方面参与细胞过程增添了另一层。

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is 5 - 15 ng/mL.

Rat

P. pastoris

N-His

P28576-2/NP_036933.2 (S87-R196)

25266  [NCBI]

RSIEEAIPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATSNLNPDHREEETGRRRESGKKRK

17-22 kDa & 25-45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.