PDGF-CC 蛋白, Human (HEK293)

PDGF-CC Protein, Human (HEK293) is a member of the PDGF family, binds to PDGFR-α and PDGFR-β and acts as potent angiogenic factor.

PDGF-CC是PDGF家族中相对较新的成员，在不同类型的细胞中大量表达，例如肿瘤细胞、内皮细胞(EC)、血管平滑肌细胞(VSMC)、周细胞、成纤维细胞和巨噬细胞。PDGF-CC 与 PDGFR-α 和 PDGFR-β 结合，它们也由许多不同的细胞类型表达，包括肿瘤细胞、VSMC、周细胞和成纤维细胞，所有这些细胞在血管生成中都发挥着重要作用。PDGF-CC 是一种有效的血管生成因子^[1]。

The ED₅₀ is <1 ng/mL as measured by 3T3 cells.

Human

HEK293

Tag Free

Q9NRA1-1 (V235-G345)

56034  [NCBI]

VVDLNLLTEEVRLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRCGGNCACCLHNCNECQCVPSKVTKKYHEVLQLRPKTGVRGLHKSLTDVALEHHEECDCVCRGSTGG

15-19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zheng L, et al. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently. Oncotarget. 2016 Nov 22;7(47):77902-77915.  [Content Brief]