2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. Peroxiredoxin-2/PRDX2 蛋白, Mouse (sf9, His)

Peroxiredoxin-2/PRDX2 蛋白, Mouse (sf9, His)

目录号: HY-P73712
产品使用指南

Peroxiredoxin-2/PRDX2 蛋白是一种硫醇特异性过氧化物酶，可有效减少过氧化氢和有机氢过氧化物，保护细胞免受氧化应激并维持氧化还原稳态。它还充当过氧化氢介导的信号传导的传感器，调节细胞对氧化应激的反应。Peroxiredoxin-2/PRDX2 蛋白, Mouse (sf9, His) 是重组的 Peroxiredoxin-2/PRDX2 蛋白，由 Sf9 insect cells 表达，带有 N-His 标签。

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描述

Peroxiredoxin-2/PRDX2 protein is a thiol-specific peroxidase that effectively reduces hydrogen peroxide and organic hydroperoxides, protecting cells from oxidative stress and maintaining redox homeostasis. It also acts as a sensor for hydrogen peroxide-mediated signaling, regulating cellular responses to oxidative stress. Peroxiredoxin-2/PRDX2 Protein, Mouse (sf9, His) is the recombinant mouse-derived Peroxiredoxin-2/PRDX2 protein, expressed by Sf9 insect cells , with N-His labeled tag.
研究背景

Peroxiredoxin-2/PRDX2 蛋白作为硫醇特异性过氧化物酶发挥作用，擅长分别催化过氧化氢和有机氢过氧化物还原为水和醇。这种蛋白质通过有效地解毒各种过氧化物，在细胞保护免受氧化应激方面发挥着至关重要的作用，对维持细胞氧化还原稳态有显着贡献。此外，PRDX2 作为过氧化氢介导的信号事件的传感器，暗示其参与调节细胞对氧化应激的反应。此外，它可能通过调节细胞内H(2)O(2) 浓度参与生长因子和肿瘤坏死因子-α 的信号级联反应，表明其对与细胞生长和炎症相关的信号传导途径具有潜在影响。
种属

Mouse
表达系统

Sf9 insect cells
标签

N-His
蛋白编号

Q61171/NP_035693.3 (M1-N198)
基因 ID
蛋白结构
N-term
His
Peroxiredoxin-2/PRDX2 (M1-N198)
Accession # Q61171/NP_035693.3
C-term
同用名
Peroxiredoxin-2; NKEF-B; TSA; PRDX2; TDPX1
分子量

Approximately 24.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Peroxiredoxin-2/PRDX2 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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