PGK1 蛋白, Human (HEK293, His)

The PGK1 protein plays a key role in the glycolysis pathway by catalyzing one of two ATP-generating reactions, promoting the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate. In addition to its glycolytic function, PGK1 appears to act as a cofactor protein for polymerase α, playing a role in primer recognition. PGK1 Protein, Human (HEK293, His) is the recombinant human-derived PGK1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PGK1 Protein, Human (HEK293, His) is 416 a.a., with molecular weight of 46-50 kDa.

磷酸甘油酸激酶 1 (PGK1) 通过催化 1,3-二磷酸甘油酸可逆转化为 3-磷酸甘油酸，在糖酵解中发挥着至关重要的作用，从而有助于 ATP 的产生。除了在糖酵解能量代谢中的既定作用之外，PGK1 还被认为可以作为辅助因子蛋白发挥作用，特别是与聚合酶 α 相关时，它充当引物识别蛋白。此外，PGK1 可能在精子活力中发挥作用，表明其对细胞过程的潜在贡献超出了其经典的糖酵解功能。PGK1 的多方面作用强调了其在细胞生物能量学中的重要性，并表明其在 DNA 复制和精子活力等细胞过程中具有其他功能。

Measured by NADH production in a reaction coupled with GAPDH. The specific activity is ≤115554.662 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

P00558-1 (S2-I417)

5230  [NCBI]

SLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

Approximately 46-50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH 8.0 or 50 mM Tris, 300 mM NaCl, pH 8.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.