PKM2 蛋白, Human (420 a.a, His)

PKM2 Protein, Human (420 a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways^[1].

丙酮酸激酶 M2 (PKM2) 是一种多功能蛋白，仅在胚胎和成体分裂/肿瘤细胞中表达。PKM2是一种普遍存在的原型酶，在胚胎阶段存在于所有组织中，并在发育过程中逐渐被特定组织中的其他同工酶形式所取代。尽管 PKM2 的主要功能是分解代谢葡萄糖，但它也参与许多非糖酵解途径，影响细胞生理学^[1]。

Measured by its ability to couple reaction catalyzed by PKM2 and LDH, detect the consumption of NADH by pyruvate, the product of phosphate (enol) pyruvate monosodium salt hydrate (PEP). Specific activity is ≥12.13 pmol/min/μg was measured under the conditions described.

Human

E. coli

His

P14618-1 (L61-E480)

5315  [NCBI]

LKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQE

Approximately 55 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 50 Mm Tris, 1 mM EDTA, trace Imidazole and DTT, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Gupta V, et, al. Human pyruvate kinase M2: a multifunctional protein. Protein Sci. 2010 Nov;19(11):2031-44.  [Content Brief]