PON3 蛋白, Human (S50N, sf9, His)

PON3 is less active against organophosphates and aromatic carboxylic acid esters and is particularly capable of rapidly hydrolyzing lactones, including statin precursors such as lovastatin. It is capable of hydrolyzing aromatic lactones and specific 5- or 6-membered ring lactones, but is less efficient for simple or polar substituted lactones. PON3 Protein, Human (S50N, sf9, His) is the recombinant human-derived PON3 protein, expressed by Sf9 insect cells , with N-His labeled tag.

PON3 对有机磷酸酯 paraxon 和芳香族羧酸酯表现出低活性。值得注意的是，它表现出内酯的快速水解作用，包括洛伐他汀等他汀类药物前体。此外，PON3能够水解芳香族内酯和具有脂肪族取代基的5或6元环内酯，但它不能有效地水解简单内酯或具有极性取代基的内酯。这些底物特异性突出了 PON3 在某些酯化合物和内酯代谢中的作用，提供了对其酶活性和药物代谢潜在影响的见解。

Human

Sf9 insect cells

N-His

Q15166-1 (M1-L354)

5446  [NCBI]

Approximately 45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 1 mM CaCl₂, 10% Glycerol, 0.1% DDM.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.