PPAR-gamma 蛋白, Human (P37231-2, His)

The PPAR gamma protein is a nuclear receptor that binds to peroxisome proliferators and is activated upon ligand binding to specific PPREs on DNA. It regulates target gene transcription and controls fatty acid metabolism. PPAR-gamma Protein, Human (P37231-2, His) is the recombinant human-derived PPAR-gamma protein, expressed by E. coli , with C-6*His labeled tag.

PPAR γ 蛋白是一种核受体，可与降血脂药物和脂肪酸等过氧化物酶体增殖物结合。配体激活后，核受体与 DNA 上的特定 PPAR 反应元件 (PPRE) 相互作用，调节酰基辅酶 A 氧化酶等靶基因的转录，从而控制脂肪酸的过氧化物酶体 β 氧化途径。它作为脂肪细胞分化和葡萄糖稳态的关键调节剂发挥着关键作用。此外，PPAR γ 通过抑制 NF-κ-B 介导的促炎症反应，充当肠道稳态的关键调节剂。在心血管昼夜节律的背景下，它调节血管中 BMAL1 的转录。此外，为了应对微生物感染，特别是结核分枝杆菌或其脂蛋白 LpqH 治疗，PPAR γ 调节 MAPK p38 的磷酸化和 IL-6 的产生，表明其参与微生物挑战期间的免疫反应。

Human

E. coli

C-6*His

P37231-2 (E207-Y477)

5468  [NCBI]

ESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY

Approximately 31 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.