PPIC 蛋白, Human (Trx-His)

PPIC protein is a peptidyl-prolyl cis-trans isomerase (PPIase) that actively catalyzes the cis-trans isomerization of prolyl imide peptide bonds, which is a key step in protein folding. Its role in promoting conformational changes ensures correct folding and maturation of proteins, contributing to cellular proteostasis. PPIC Protein, Human (Trx-His) is the recombinant human-derived PPIC protein, expressed by E. coli , with N-Trx, N-6*His labeled tag. The total length of PPIC Protein, Human (Trx-His) is 152 a.a., with molecular weight of ~34.0 kDa.

PPIC 蛋白作为肽基-脯氨酰顺反异构酶 (PPIase) 出现，积极促进寡肽中脯氨酸酰亚胺肽键的顺反异构化，这是复杂的蛋白质折叠过程中的关键步骤。通过催化这些构象变化，PPIC 预计将在确保蛋白质正确折叠和成熟方面发挥关键作用，从而有助于细胞蛋白质稳态。PPIC 异构化脯氨酸酰亚胺肽键的能力强调了其在调节多肽结构动力学、最终影响其功能完整性方面的重要性。这种多功能酶活性使 PPIC 成为细胞生理学的关键参与者，强调需要进一步探索以揭示 PPIC 对复杂的蛋白质折叠世界做出积极贡献的特定分子机制和细胞途径。

Measured by its ability to convert the substrate, Suc-AAPF-pNA, from Cis to Trans formation. The specific activity is 776.34 pmol/min/μg.

Human

E. coli

N-Trx;N-6*His

P45877 (K31-D182)

5480  [NCBI]

KRGPSVTAKVFFDVRIGDKDVGRIVIGLFGKVVPKTVENFVALATGEKGYGYKGSKFHRVIKDFMIQGGDITTGDGTGGVSIYGETFPDENFKLKHYGIGWVSMANAGPDTNGSQFFITLTKPTWLDGKHVVFGKVIDGMTVVHSIELQATD

Approximately 34.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.