PPID 蛋白, Human (His)

PPID protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and contributes to protein folding. It serves as a co-chaperone in the HSP90 complex, contributing to dynamic regulation and may favor the estrogen receptor complex. PPID Protein, Human (His) is the recombinant human-derived PPID protein, expressed by E. coli , with N-6*His, C-6*His labeled tag. The total length of PPID Protein, Human (His) is 370 a.a., with molecular weight of ~43.85 kDa.

PPID 蛋白作为肽基-脯氨酰顺反异构酶 (PPIase)，催化寡肽中脯氨酸酰亚胺肽键的顺反异构化，并可能有助于蛋白质折叠过程。PPID 被提议在 HSP90 复合物中充当辅助伴侣，特别是在未连接的类固醇受体杂复合物中，有助于 HSP90-辅助伴侣-受体复合物的动态调节。这种参与表明在受体活性的组织特异性控制中发挥作用，与糖皮质激素受体复合物相比，雌激素受体复合物具有潜在的偏好。此外，PPID 可能参与受体从细胞质到细胞核的细胞质动力蛋白依赖性运动。值得注意的是，它通过抑制 MYB 的 DNA 结合活性来调节 MYB，并通过促进 AHR:ARNT 二聚体的形成在 AHR 信号传导中发挥作用，独立于 HSP90，但需要伴侣活性。此外，PPID 还参与调节紫外线辐射诱导的细胞凋亡，并促进间变性淋巴瘤激酶阳性间变性大细胞淋巴瘤 (ALK+ ALCL) 细胞系的细胞活力。在微生物感染的情况下，PPID 可能与丙型肝炎病毒 (HCV) 的复制和释放有关。

Measured by its ability to convert the substrate, Suc-AAPF-pNA, from Cis to Trans formation. The specific activity is 904.66 pmol/min/μg, as measured under the described conditions.

Human

E. coli

N-6*His;C-6*His

Q08752 (M1-A370)

5481  [NCBI]

MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

Approximately 43.85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.