PPM1A 蛋白, Human (His)

PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.

PPM1A 是一种具有广泛特异性的酶，在多种信号通路中充当负调节因子。它通过使 SMAD2 和 SMAD3 去磷酸化，在 TGF-β 信号传导减弱中发挥关键作用。这种酶促作用导致 SMAD2 和 SMAD3 从 SMAD4 解离、它们的核输出，并最终终止 TGF-β 介导的信号传导。此外，PPM1A 使 PRKAA1 和 PRKAA2 去磷酸化，有助于细胞过程的调节。其调节功能的另一个重要方面涉及终止 TNF-α 介导的 NF-κ-B 激活。在这种情况下，PPM1A 使 IKBKB/IKKB 去磷酸化和失活，从而导致 NF-kappa-B 信号传导的下调。

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 1060.04 nmol/min/mg.

Human

E. coli

C-6*His

P35813-1 (G2-W382)

5494  [NCBI]

GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW

Approximately 45.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.