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  3. PRADC1 蛋白, Human (HEK293, His)

PRADC1 蛋白, Human (HEK293, His)

目录号: HY-P7694
产品使用指南

PRADC1 蛋白, Human (HEK293, His)是一种由 HEK293 细胞表达产生的,带有 His 标签的重组人类 PRADC1。PRADC1 是一种代谢反应分泌蛋白,可调节身体活动和肥胖。

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描述

PRADC1 Protein, Human (HEK293, His) is a recombinant human protease-associated domain-containing 1 (PRADC1) produced in HEK293 cells, with His tag. PRADC1 is a metabolic-responsive secretory protein that modulates physical activity and adiposity[1].

研究背景

PRADC1(也称为 PAP21)是一种 ∼30-kDa N-糖基化分泌蛋白,具有蛋白酶相关结构域。PRADC1 是能量代谢的分泌调节因子[1]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9BSG0 (H22-W188)

基因 ID

84279  [NCBI]

蛋白结构
N-term
PRADC1 (H22-W188)
Accession # Q9BSG0
6*His
C-term
同用名
rHuPRADC1, His; Protease-associated domain-containing protein 1; PRADC1; C2orf7
氨基酸序列

HGFRIHDYLYFQVLSPGDIRYIFTATPAKDFGGIFHTRYEQIHLVPAEPPEACGELSNGFFIQDQIALVERGGCSFLSKTRVVQEHGGRAVIISDNAVDNDSFYVEMIQDSTQRTADIPALFLLGRDGYMIRRSLEQHGLPWAIISIPVNVTSIPTFELLQPPWTFWHHHHHH

分子量

Approximately 28.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

PRADC1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PRADC1 Protein, Human (HEK293, His)
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