PRAK/MAPKAPK5 蛋白, Human (sf9, His-GST)

PRAK/MAPKAPK5 protein, a serine/threonine kinase, responds to cellular stress and pro-inflammatory cytokines and acts as a tumor suppressor through MAP kinases (including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta) activated by phosphorylation. Initially located in the nucleus, upon activation, it moves to the cytoplasm and phosphorylates the heat shock protein HSP27. PRAK/MAPKAPK5 Protein, Human (sf9, His-GST) is the recombinant human-derived PRAK/MAPKAPK5 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PRAK/MAPKAPK5 Protein, Human (sf9, His-GST) is 471 a.a., with molecular weight of ~35-45 kDa.

PRAK/MAPKAPK5 蛋白属于丝氨酸/苏氨酸激酶家族，具有肿瘤抑制因子的功能，并通过 MAP 激酶（包括 MAPK1/ERK、MAPK14/p38-alpha 和 MAPK11）磷酸化而被激活，从而对细胞应激和促炎细胞因子做出反应/p38-beta。虽然最初位于细胞核，但在磷酸化和激活后，它转移到细胞质。该激酶的一个值得注意的靶标是热休克蛋白 HSP27，可在生理相关位点将其磷酸化。该基因显示出两种编码不同亚型的选择性剪接转录物变体。PRAK/MAPKAPK5 表现出无处不在的表达，在小肠 (RPKM 3.1)、结肠 (RPKM 2.9) 和 25 个其他组织中具有可辨别的水平，强调其广泛参与多个器官的不同生理环境。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-Strep

Q8IW41-2/NP_003659.2 (M1-Q471)

8550  [NCBI]

Approximately 35-45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.