PRDC/GREM2 蛋白, Mouse

PRDC, encoded by the GREM2 gene, has multiple functions, including BMP and protein binding, which are critical for dorsal identity determination, embryoid body morphogenesis, and signal transduction regulation. PRDC is found extracellularly and is expressed in structures such as the cartilaginous skull, integument, intestinal smooth muscle annulus, lung, and neural tube. PRDC/GREM2 Protein, Mouse is the recombinant mouse-derived PRDC/GREM2 protein, expressed by E. coli , with tag free.

以GREM2基因为代表的PRDC是一种多功能蛋白质，具有多种功能，包括BMP结合活性、肝素结合活性和相同的蛋白质结合活性。该蛋白在多种过程中发挥着至关重要的作用，例如背部身份的确定、胚胎体形态发生和信号转导的调节。PRDC 存在于细胞外空间，在多种结构中表达，如软骨颅、珠被、肠平滑肌环层、肺和神经管。该基因的人类直系同源物与牙齿发育不全有关，强调了其在发育过程中的重要性。跨多个组织（包括卵巢和肺）的广泛表达模式强调了 PRDC 在各种生理环境中的广泛影响。

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.04067 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4, corresponding to a specific activity is 2.46×10⁴units/mg.

Mouse

E. coli

Tag Free

O88273/NP_035955 (R22-Q168)

23893  [NCBI]

RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQTVSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLDPPFRIKKIQKVKHCRCMSVNLSDSDKQ

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 0.01% TFA, 30% Acetonitrile, pH 4.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.