1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. PKC-nu
  5. PRKD3/nPKC nu 蛋白, Human (sf9, GST)

PRKD3/nPKC nu 蛋白, Human (sf9, GST)

目录号: HY-P73699
产品使用指南

PRKD3/nPKC nu 蛋白在瞬时二酰甘油 (DAG) 信号转化为持续生理效应的过程中充当介质,在 PKC 下游发挥作用。此外,它在抵抗氧化应激方面发挥着至关重要的作用,表明它参与细胞对环境挑战的反应。PRKD3/nPKC nu 蛋白, Human (sf9, GST) 是重组的 PRKD3/nPKC nu 蛋白,由 Sf9 insect cells 表达,带有 GST 标签。

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描述

PRKD3/nPKC nu protein acts as a mediator in the conversion of transient diacylglycerol (DAG) signals into sustained physiological effects, functioning downstream of PKC.Additionally, it plays a crucial role in conferring resistance to oxidative stress, demonstrating its involvement in cellular responses to environmental challenges.PRKD3/nPKC nu Protein, Human (sf9, GST) is the recombinant human-derived PRKD3/nPKC nu protein, expressed by Sf9 insect cells , with GST labeled tag.

研究背景

蛋白激酶 D3 (PRKD3) 也称为新型蛋白激酶 C nu (nPKC nu),通过将瞬时二酰基甘油 (DAG) 信号转化为持续的生理效应,在蛋白激酶 C (PKC) 下游发挥作用,从而在细胞信号传导中发挥关键作用。这种激酶调节信号级联的能力表明它参与依赖于对二酰基甘油的长期反应的多种细胞过程。此外,PRKD3/nPKC nu 与赋予氧化应激抵抗力有关,表明其在针对活性氧和相关应激源的细胞防御机制中的潜在作用(通过相似性)。PRKD3/nPKC nu 的多方面功能强调了其在信号转导中的重要性,表明其对细胞适应和生存以应对各种环境挑战的贡献。

种属

Human

表达系统

Sf9 insect cells

标签

GST

蛋白编号

O94806 (S2-P890)

基因 ID
同用名
Serine/threonine-protein kinase D3; nPKC-nu; PRKD3; EPK2; PRKCN
分子量

Approximately 126.7 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PRKD3/nPKC nu Protein, Human (sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PRKD3/nPKC nu Protein, Human (sf9, GST)
目录号:
HY-P73699
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