Profilin-4 蛋白, Human (His)

The profilin-4 protein is critical for male fertility and plays an important role in manchette development and acrosome biogenesis during spermatogenesis. It has binding affinity for a variety of phospholipids, including PtdIns(3)P, PtdIns(4,5)P2, PtdIns(4)P, and PA. Profilin-4 Protein, Human (His) is the recombinant human-derived Profilin-4 protein, expressed by E. coli , with N-His labeled tag. The total length of Profilin-4 Protein, Human (His) is 129 a.a., with molecular weight of ~14 kDa.

Profilin-4 蛋白在男性生育能力中发挥着至关重要的作用，对于精子发生过程中的 manchette 发育和顶体生物发生至关重要。该蛋白在体外表现出与各种磷脂的结合亲和力，包括磷脂酰肌醇 3-磷酸 (PtdIns(3)P)、磷脂酰肌醇 4,5-二磷酸 (PtdIns(4,5)P2)、磷脂酰肌醇 4-磷酸 (PtdIns(4)P) ）和磷脂酸（PA）。值得注意的是，与 Profilin 家族的其他成员不同，Profilin-4 在体外不与肌动蛋白结合。这些独特的特性强调了其在男性生殖生理学复杂过程中的特殊功能，特别是在精子成熟过程中曼切特和顶体的发育中。

Human

E. coli

N-6*His

Q8NHR9 (M1-S129)

375189  [NCBI]

MSHLQSLLLDTLLGTKHVDSAALIKIQERSLCVASPGFNVTPSDVRTLVNGFAKNPLQARREGLYFKGKDYRCVRADEYSLYAKNENTGVVVVKTHLYLLVATYTEGMYPSICVEATESLGDYLRKKGS

Approximately 14 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.