PRSS3/Trypsin-3 蛋白, Human (HEK293, His)

PRSS3/Trypsin-3 is a digestive protease with specific substrate preference that cleaves proteins after Arg residues. This enzymatic activity is combined with its ability to exhibit proteolytic effects on Kunitz-type trypsin inhibitors. PRSS3/Trypsin-3 Protein, Human (HEK293, His) is the recombinant human-derived PRSS3/Trypsin-3 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of PRSS3/Trypsin-3 Protein, Human (HEK293, His) is 232 a.a., with molecular weight of ~33 kDa.

PRSS3，也称为胰蛋白酶-3，是一种具有独特底物特异性的消化蛋白酶，因为它优先在精氨酸残基后裂解蛋白质。这种类似胰蛋白酶的酶通过将膳食蛋白质分解成更小的肽，在消化过程中发挥着至关重要的作用。值得注意的是，PRSS3 对 Kunitz 型胰蛋白酶抑制剂表现出蛋白水解活性，表明其参与与内源性蛋白酶抑制剂的调节相互作用。该酶在特定位点切割蛋白质的能力强调了其在蛋白质消化中的重要性，并且其与胰蛋白酶抑制剂的相互作用突出了其在细胞环境内蛋白酶活性的复杂平衡中的作用。正在进行的研究可能会进一步揭示 PRSS3 在消化生理学中的生理意义和调节功能。

Measured by its ability to cleave a peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm. The specific activity is 3774.8148 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His;C-6*His

P35030-3 (V16-S247)

5646  [NCBI]

VPFDDDDKIVGGYTCEENSLPYQVSLNSGSHFCGGSLISEQWVVSAAHCYKTRIQVRLGEHNIKVLEGNEQFINAAKIIRHPKYNRDTLDNDIMLIKLSSPAVINARVSTISLPTTPPAAGTECLISGWGNTLSFGADYPDELKCLDAPVLTQAECKASYPGKITNSMFCVGFLEGGKDSCQRDSGGPVVCNGQLQGVVSWGHGCAWKNRPGVYTKVYNYVDWIKDTIAANS

Approximately 33 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.