PTGDS 蛋白, Human (HEK293, C-His)

The PTGDS protein serves a multifaceted role in various physiological processes. It catalyzes the conversion of PGH2 to PGD2, a prostaglandin with implications in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Beyond its involvement in these vascular functions, PTGDS plays a crucial role in diverse central nervous system (CNS) activities, including sedation, NREM sleep, and PGE2-induced allodynia, and may exhibit an anti-apoptotic function in oligodendrocytes. Notably, PTGDS has a broad substrate-binding capacity, interacting with small non-substrate lipophilic molecules such as biliverdin, bilirubin, retinal, retinoic acid, and thyroid hormone. This suggests its potential role as a scavenger for harmful hydrophobic molecules and as a transporter for secretory retinoids and thyroid hormones. Additionally, PTGDS is implicated in the development and maintenance of various blood barriers, including the blood-brain, blood-retina, blood-aqueous humor, and blood-testis barriers, indicating its significance in barrier function. Furthermore, it plays essential roles in the maturation and maintenance of the central nervous system and the male reproductive system, as well as participates in PLA2G3-dependent maturation of mast cells, further emphasizing its intricate involvement in diverse biological processes. PTGDS Protein, Human (HEK293, C-His) is the recombinant human-derived PTGDS protein, expressed by HEK293 , with C-10*His labeled tag. The total length of PTGDS Protein, Human (HEK293, C-His) is 168 a.a., with molecular weight of ~21 kDa.

PTGDS 蛋白在各种生理过程中发挥多方面的作用。它催化 PGH2 转化为 PGD2，后者是一种前列腺素，与平滑肌收缩/松弛有关，也是血小板聚集的有效抑制剂。除了参与这些血管功能之外，PTGDS 在多种中枢神经系统 (CNS) 活动中发挥着至关重要的作用，包括镇静、NREM 睡眠和 PGE2 诱导的异常性疼痛，并且可能在少突胶质细胞中表现出抗凋亡功能。值得注意的是，PTGDS 具有广泛的底物结合能力，可与胆绿素、胆红素、视黄醛、视黄酸和甲状腺激素等小非底物亲脂性分子相互作用。这表明它具有作为有害疏水分子的清除剂以及作为分泌性视黄醇和甲状腺激素的转运蛋白的潜在作用。此外，PTGDS还参与多种血液屏障的形成和维持，包括血脑、血-视网膜、血-房水和血-睾丸屏障，表明其在屏障功能中的重要性。此外，它在中枢神经系统和男性生殖系统的成熟和维持中发挥着重要作用，并参与肥大细胞的PLA2G3依赖性成熟，进一步强调了它在多种生物过程中的复杂参与。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-10*His

P41222 (A23-Q190)

5730  [NCBI]

APEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKKAALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSVVETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQTDKCMTEQ

Approximately 21 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.